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. 1988 Jun 15;252(3):795–799. doi: 10.1042/bj2520795

Mannose 6-phosphate/insulin-like growth factor II-binding proteins in human serum and urine. Their relation to the mannose 6-phosphate/insulin-like growth factor II receptor.

C Causin 1, A Waheed 1, T Braulke 1, U Junghans 1, P Maly 1, R E Humbel 1, K von Figura 1
PMCID: PMC1149217  PMID: 2458716

Abstract

Human serum and urine contain polypeptides which bind mannose 6-phosphate (M6P) and insulin-like growth factor II (IGF II) and crossreact with antibodies against the M6P/IGF II receptor. These polypeptides are considered to be fragments of the M6P/IGF II receptor. The major Mr approx. 205,000 fragment in serum and urine is about 10 kDa smaller in size than the membrane-associated receptor and is accompanied by minor forms with Mr values ranging from 104,000 to 180,000. The presence of receptor fragments in biological fluids indicates that shedding is one of the mechanisms contributing to the turnover of the M6P/IGF II receptor and that receptor fragments are part of the heterogenous group of serum proteins whic bind IGF II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Conary J. T., Lorkowski G., Schmidt B., Pohlmann R., Nagel G., Meyer H. E., Krentler C., Cully J., Hasilik A., von Figura K. Genetic heterogeneity of steroid sulfatase deficiency revealed with cDNA for human steroid sulfatase. Biochem Biophys Res Commun. 1987 Apr 29;144(2):1010–1017. doi: 10.1016/s0006-291x(87)80064-5. [DOI] [PubMed] [Google Scholar]
  2. Creek K. E., Sly W. S. Biosynthesis and turnover of the phosphomannosyl receptor in human fibroblasts. Biochem J. 1983 Aug 15;214(2):353–360. doi: 10.1042/bj2140353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Frackelton A. R., Jr, Szaro R. P., Weltman J. K. A galactosidase immunosorbent test for carcinoembryonic antigen. Cancer Res. 1976 Aug;36(8):2845–2849. [PubMed] [Google Scholar]
  4. Geuze H. J., Slot J. W., Strous G. J., Hasilik A., Von Figura K. Ultrastructural localization of the mannose 6-phosphate receptor in rat liver. J Cell Biol. 1984 Jun;98(6):2047–2054. doi: 10.1083/jcb.98.6.2047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kiess W., Greenstein L. A., White R. M., Lee L., Rechler M. M., Nissley S. P. Type II insulin-like growth factor receptor is present in rat serum. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7720–7724. doi: 10.1073/pnas.84.21.7720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Morgan D. O., Edman J. C., Standring D. N., Fried V. A., Smith M. C., Roth R. A., Rutter W. J. Insulin-like growth factor II receptor as a multifunctional binding protein. Nature. 1987 Sep 24;329(6137):301–307. doi: 10.1038/329301a0. [DOI] [PubMed] [Google Scholar]
  8. Morgan D. O., Roth R. A. Plate binding assay for monoclonal anti-receptor antibodies. Endocrinology. 1985 Mar;116(3):1224–1226. doi: 10.1210/endo-116-3-1224. [DOI] [PubMed] [Google Scholar]
  9. Moses A. C., Nessley S. P., Cohen K. L., Rechler M. M. Specific binding of a somatomedin-like polypeptide in rat serum depends on growth hormone. Nature. 1976 Sep 9;263(5573):137–140. doi: 10.1038/263137a0. [DOI] [PubMed] [Google Scholar]
  10. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  11. Rinderknecht E., Humbel R. E. Amino-terminal sequences of two polypeptides from human serum with nonsuppressible insulin-like and cell-growth-promoting activities: evidence for structural homology with insulin B chain. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4379–4381. doi: 10.1073/pnas.73.12.4379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sahagian G. G., Steer C. J. Transmembrane orientation of the mannose 6-phosphate receptor in isolated clathrin-coated vesicles. J Biol Chem. 1985 Aug 15;260(17):9838–9842. [PubMed] [Google Scholar]
  13. Sahagian G. G. The mannose 6-phosphate receptor: function, biosynthesis and translocation. Biol Cell. 1984;51(2):207–214. doi: 10.1111/j.1768-322x.1984.tb00300.x. [DOI] [PubMed] [Google Scholar]
  14. Stein M., Meyer H. E., Hasilik A., von Figura K. 46-kDa mannose 6-phosphate-specific receptor: purification, subunit composition, chemical modification. Biol Chem Hoppe Seyler. 1987 Aug;368(8):927–936. doi: 10.1515/bchm3.1987.368.2.927. [DOI] [PubMed] [Google Scholar]
  15. Zapf J., Walter H., Froesch E. R. Radioimmunological determination of insulinlike growth factors I and II in normal subjects and in patients with growth disorders and extrapancreatic tumor hypoglycemia. J Clin Invest. 1981 Nov;68(5):1321–1330. doi: 10.1172/JCI110379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. von Figura K., Gieselmann V., Hasilik A. Mannose 6-phosphate-specific receptor is a transmembrane protein with a C-terminal extension oriented towards the cytosol. Biochem J. 1985 Jan 15;225(2):543–547. doi: 10.1042/bj2250543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. von Figura K., Hasilik A. Lysosomal enzymes and their receptors. Annu Rev Biochem. 1986;55:167–193. doi: 10.1146/annurev.bi.55.070186.001123. [DOI] [PubMed] [Google Scholar]

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