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. 1988 Jun 15;252(3):901–904. doi: 10.1042/bj2520901

Phosphorylation of the subunits of cytochrome b-245 upon triggering of the respiratory burst of human neutrophils and macrophages.

R C Garcia 1, A W Segal 1
PMCID: PMC1149233  PMID: 2844158

Abstract

Cytochrome b-245, the only clearly identified component of the microbicidal oxidase system of phagocytes, is a heterodimer consisting of a 23 kDa (alpha) and a 76-92 kDa (beta) subunit. This study was conducted to examine whether, in common with a number of proteins, the subunits of the cytochrome were phosphorylated upon activation of the oxidase. Both subunits were phosphorylated after activation of neutrophils or macrophages with phorbol myristate acetate or a phagocytic stimulus, although the time course of this process did not parallel that of the oxidase. Phosphorylation of these proteins was normal in cells from two patients with autosomal recessive chronic granulomatous disease, in whom phosphorylation of a 47 kDa protein is defective.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. C., Babior B. M. Endogenous protein phosphorylation by resting and activated human neutrophils. Blood. 1983 Feb;61(2):333–340. [PubMed] [Google Scholar]
  2. Cross A. R., Higson F. K., Jones O. T., Harper A. M., Segal A. W. The enzymic reduction and kinetics of oxidation of cytochrome b-245 of neutrophils. Biochem J. 1982 May 15;204(2):479–485. doi: 10.1042/bj2040479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cross A. R., Jones O. T., Garcia R., Segal A. W. The association of FAD with the cytochrome b-245 of human neutrophils. Biochem J. 1982 Dec 15;208(3):759–763. doi: 10.1042/bj2080759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Garcia R. C., Cross A. R., Segal A. W. The development of cytochrome b-245 in maturing human macrophages. Biochem J. 1986 Nov 1;239(3):647–651. doi: 10.1042/bj2390647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harper A. M., Dunne M. J., Segal A. W. Purification of cytochrome b-245 from human neutrophils. Biochem J. 1984 Apr 15;219(2):519–527. doi: 10.1042/bj2190519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heyworth P. G., Segal A. W. Further evidence for the involvement of a phosphoprotein in the respiratory burst oxidase of human neutrophils. Biochem J. 1986 Nov 1;239(3):723–731. doi: 10.1042/bj2390723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmes B., Page A. R., Good R. A. Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function. J Clin Invest. 1967 Sep;46(9):1422–1432. doi: 10.1172/JCI105634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Parkos C. A., Allen R. A., Cochrane C. G., Jesaitis A. J. Purified cytochrome b from human granulocyte plasma membrane is comprised of two polypeptides with relative molecular weights of 91,000 and 22,000. J Clin Invest. 1987 Sep;80(3):732–742. doi: 10.1172/JCI113128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Royer-Pokora B., Kunkel L. M., Monaco A. P., Goff S. C., Newburger P. E., Baehner R. L., Cole F. S., Curnutte J. T., Orkin S. H. Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location. Nature. 1986 Jul 3;322(6074):32–38. doi: 10.1038/322032a0. [DOI] [PubMed] [Google Scholar]
  11. Schneider C., Zanetti M., Romeo D. Surface-reactive stimuli selectively increase protein phosphorylation in human neutrophils. FEBS Lett. 1981 May 5;127(1):4–8. doi: 10.1016/0014-5793(81)80327-4. [DOI] [PubMed] [Google Scholar]
  12. Segal A. W. Absence of both cytochrome b-245 subunits from neutrophils in X-linked chronic granulomatous disease. Nature. 1987 Mar 5;326(6108):88–91. doi: 10.1038/326088a0. [DOI] [PubMed] [Google Scholar]
  13. Segal A. W., Cross A. R., Garcia R. C., Borregaard N., Valerius N. H., Soothill J. F., Jones O. T. Absence of cytochrome b-245 in chronic granulomatous disease. A multicenter European evaluation of its incidence and relevance. N Engl J Med. 1983 Feb 3;308(5):245–251. doi: 10.1056/NEJM198302033080503. [DOI] [PubMed] [Google Scholar]
  14. Segal A. W., Dorling J., Coade S. Kinetics of fusion of the cytoplasmic granules with phagocytic vacuoles in human polymorphonuclear leukocytes. Biochemical and morphological studies. J Cell Biol. 1980 Apr;85(1):42–59. doi: 10.1083/jcb.85.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Segal A. W., Heyworth P. G., Cockcroft S., Barrowman M. M. Stimulated neutrophils from patients with autosomal recessive chronic granulomatous disease fail to phosphorylate a Mr-44,000 protein. Nature. 1985 Aug 8;316(6028):547–549. doi: 10.1038/316547a0. [DOI] [PubMed] [Google Scholar]
  16. Segal A. W., Jones O. T. Absence of cytochrome b reduction in stimulated neutrophils from both female and male patients with chronic granulomatous disease. FEBS Lett. 1980 Jan 28;110(1):111–114. doi: 10.1016/0014-5793(80)80035-4. [DOI] [PubMed] [Google Scholar]
  17. Teahan C., Rowe P., Parker P., Totty N., Segal A. W. The X-linked chronic granulomatous disease gene codes for the beta-chain of cytochrome b-245. 1987 Jun 25-Jul 1Nature. 327(6124):720–721. doi: 10.1038/327720a0. [DOI] [PubMed] [Google Scholar]

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