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. 1988 Aug 1;253(3):932–933. doi: 10.1042/bj2530932

The deoxyribose assay: an assay both for 'free' hydroxyl radical and for site-specific hydroxyl radical production.

J M Gutteridge, B Halliwell
PMCID: PMC1149395  PMID: 2845941

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Czapski G., Goldstein S. When do metal complexes protect the biological system from superoxide toxicity and when do they enhance it? Free Radic Res Commun. 1986;1(3):157–161. doi: 10.3109/10715768609083147. [DOI] [PubMed] [Google Scholar]
  2. Gutteridge J. M. Ferrous-salt-promoted damage to deoxyribose and benzoate. The increased effectiveness of hydroxyl-radical scavengers in the presence of EDTA. Biochem J. 1987 May 1;243(3):709–714. doi: 10.1042/bj2430709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gutteridge J. M. Reactivity of hydroxyl and hydroxyl-like radicals discriminated by release of thiobarbituric acid-reactive material from deoxy sugars, nucleosides and benzoate. Biochem J. 1984 Dec 15;224(3):761–767. doi: 10.1042/bj2240761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gutteridge J. M. Thiobarbituric acid-reactivity following iron-dependent free-radical damage to amino acids and carbohydrates. FEBS Lett. 1981 Jun 15;128(2):343–346. doi: 10.1016/0014-5793(81)80113-5. [DOI] [PubMed] [Google Scholar]
  5. Halliwell B., Grootveld M., Gutteridge J. M. Methods for the measurement of hydroxyl radicals in biomedical systems: deoxyribose degradation and aromatic hydroxylation. Methods Biochem Anal. 1988;33:59–90. doi: 10.1002/9780470110546.ch2. [DOI] [PubMed] [Google Scholar]
  6. Halliwell B., Gutteridge J. M., Aruoma O. I. The deoxyribose method: a simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem. 1987 Aug 15;165(1):215–219. doi: 10.1016/0003-2697(87)90222-3. [DOI] [PubMed] [Google Scholar]
  7. Halliwell B., Gutteridge J. M. Formation of thiobarbituric-acid-reactive substance from deoxyribose in the presence of iron salts: the role of superoxide and hydroxyl radicals. FEBS Lett. 1981 Jun 15;128(2):347–352. doi: 10.1016/0014-5793(81)80114-7. [DOI] [PubMed] [Google Scholar]
  8. Halliwell B., Gutteridge J. M. Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Arch Biochem Biophys. 1986 May 1;246(2):501–514. doi: 10.1016/0003-9861(86)90305-x. [DOI] [PubMed] [Google Scholar]
  9. Halliwell B., Gutteridge J. M. The importance of free radicals and catalytic metal ions in human diseases. Mol Aspects Med. 1985;8(2):89–193. doi: 10.1016/0098-2997(85)90001-9. [DOI] [PubMed] [Google Scholar]
  10. Halliwell B. Superoxide-dependent formation of hydroxyl radicals in the presence of iron chelates: is it a mechanism for hydroxyl radical production in biochemical systems? FEBS Lett. 1978 Aug 15;92(2):321–326. doi: 10.1016/0014-5793(78)80779-0. [DOI] [PubMed] [Google Scholar]
  11. McCord J. M., Day E. D., Jr Superoxide-dependent production of hydroxyl radical catalyzed by iron-EDTA complex. FEBS Lett. 1978 Feb 1;86(1):139–142. doi: 10.1016/0014-5793(78)80116-1. [DOI] [PubMed] [Google Scholar]
  12. Minotti G., Aust S. D. The role of iron in the initiation of lipid peroxidation. Chem Phys Lipids. 1987 Jul-Sep;44(2-4):191–208. doi: 10.1016/0009-3084(87)90050-8. [DOI] [PubMed] [Google Scholar]
  13. Moorhouse C. P., Halliwell B., Grootveld M., Gutteridge J. M. Cobalt(II) ion as a promoter of hydroxyl radical and possible 'crypto-hydroxyl' radical formation under physiological conditions. Differential effects of hydroxyl radical scavengers. Biochim Biophys Acta. 1985 Dec 13;843(3):261–268. doi: 10.1016/0304-4165(85)90147-3. [DOI] [PubMed] [Google Scholar]
  14. Puppo A., Halliwell B. Formation of hydroxyl radicals from hydrogen peroxide in the presence of iron. Is haemoglobin a biological Fenton reagent? Biochem J. 1988 Jan 1;249(1):185–190. doi: 10.1042/bj2490185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sutton H. C., Vile G. F., Winterbourn C. C. Radical driven Fenton reactions--evidence from paraquat radical studies for production of tetravalent iron in the presence and absence of ethylenediaminetetraacetic acid. Arch Biochem Biophys. 1987 Aug 1;256(2):462–471. doi: 10.1016/0003-9861(87)90603-5. [DOI] [PubMed] [Google Scholar]
  16. Tadolini B., Cabrini L. On the mechanism of OH. scavenger action. Biochem J. 1988 Aug 1;253(3):931–932. doi: 10.1042/bj2530931. [DOI] [PMC free article] [PubMed] [Google Scholar]

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