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. 1990 Oct 1;271(1):269–272. doi: 10.1042/bj2710269

Effect of local anaesthetics on mitochondrial membrane potential in living cells.

M Grouselle 1, O Tueux 1, P Dabadie 1, D Georgescaud 1, J P Mazat 1
PMCID: PMC1149544  PMID: 2222418

Abstract

Using the laser dye rhodamine 123, we demonstrated that local anaesthetics can reach mitochondria in cell culture and reversibly decrease, or even collapse, their transmembrane potential. This effect is highly dependent on the lipid-solubility of the local anaesthetic and can be facilitated by the presence of a lipophilic anion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chazotte B., Vanderkooi G., Chignell D. Further studies on F1-ATPase inhibition by local anesthetics. Biochim Biophys Acta. 1982 Jun 18;680(3):310–316. doi: 10.1016/0005-2728(82)90144-x. [DOI] [PubMed] [Google Scholar]
  2. Chazotte B., Vanderkooi G. Multiple sites of inhibition of mitochondrial electron transport by local anesthetics. Biochim Biophys Acta. 1981 Jul;636(2):153–161. doi: 10.1016/0005-2728(81)90088-8. [DOI] [PubMed] [Google Scholar]
  3. Chen L. B. Mitochondrial membrane potential in living cells. Annu Rev Cell Biol. 1988;4:155–181. doi: 10.1146/annurev.cb.04.110188.001103. [DOI] [PubMed] [Google Scholar]
  4. Chen L. B., Summerhayes I. C., Johnson L. V., Walsh M. L., Bernal S. D., Lampidis T. J. Probing mitochondria in living cells with rhodamine 123. Cold Spring Harb Symp Quant Biol. 1982;46(Pt 1):141–155. doi: 10.1101/sqb.1982.046.01.018. [DOI] [PubMed] [Google Scholar]
  5. Dabadie P., Bendriss P., Erny P., Mazat J. P. Uncoupling effects of local anesthetics on rat liver mitochondria. FEBS Lett. 1987 Dec 21;226(1):77–82. doi: 10.1016/0014-5793(87)80554-9. [DOI] [PubMed] [Google Scholar]
  6. Emaus R. K., Grunwald R., Lemasters J. J. Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochim Biophys Acta. 1986 Jul 23;850(3):436–448. doi: 10.1016/0005-2728(86)90112-x. [DOI] [PubMed] [Google Scholar]
  7. Fayle D. R., Barritt G. J., Bygrave F. L. The effect of butacaine on adenine nucleotide binding and translocation in rat liver mitochondria. Biochem J. 1975 Jun;148(3):527–531. doi: 10.1042/bj1480527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Garlid K. D., Nakashima R. A. Studies on the mechanism of uncoupling by amine local anesthetics. Evidence for mitochondrial proton transport mediated by lipophilic ion pairs. J Biol Chem. 1983 Jul 10;258(13):7974–7980. [PubMed] [Google Scholar]
  9. Hille B. Local anesthetics: hydrophilic and hydrophobic pathways for the drug-receptor reaction. J Gen Physiol. 1977 Apr;69(4):497–515. doi: 10.1085/jgp.69.4.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson L. V., Walsh M. L., Chen L. B. Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sci U S A. 1980 Feb;77(2):990–994. doi: 10.1073/pnas.77.2.990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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