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. 1990 Nov 15;272(1):239–242. doi: 10.1042/bj2720239

Cell swelling inhibits proteolysis in perfused rat liver.

D Häussinger 1, C Hallbrucker 1, S vom Dahl 1, F Lang 1, W Gerok 1
PMCID: PMC1149682  PMID: 2264828

Abstract

Exposure of isolated single-pass-perfused rat liver to hypo-osmotic media resulted in liver cell swelling and an inhibition of release of branched-chain amino acids. Similarly, cell swelling inhibited [3H]leucine release from perfused livers from rats in which liver proteins were prelabelled in vivo by intraperitoneal injection of L-[4,5-3H]leucine 16-20 h before the experiment. The effects of cell swelling on [3H]leucine release were fully reversible. [3H]Leucine release was also inhibited when cell swelling was induced by addition of glutamine (0.5-2 mM). There was a close relationship between the inhibition of [3H]leucine release and the degree of liver cell swelling, regardless of whether cell swelling was induced by hypo-osmotic perfusion or addition of glutamine. The data suggest that the known anti-proteolytic effect of glutamine is in large part due to glutamine-induced hepatocyte swelling.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baquet A., Hue L., Meijer A. J., van Woerkom G. M., Plomp P. J. Swelling of rat hepatocytes stimulates glycogen synthesis. J Biol Chem. 1990 Jan 15;265(2):955–959. [PubMed] [Google Scholar]
  2. Graf J., Haddad P., Haeussinger D., Lang F. Cell volume regulation in liver. Ren Physiol Biochem. 1988 May-Oct;11(3-5):202–220. doi: 10.1159/000173163. [DOI] [PubMed] [Google Scholar]
  3. Häussinger D., Gerok W., Sies H. Regulation of flux through glutaminase and glutamine synthetase in isolated perfused rat liver. Biochim Biophys Acta. 1983 Jan 25;755(2):272–278. doi: 10.1016/0304-4165(83)90214-3. [DOI] [PubMed] [Google Scholar]
  4. Häussinger D., Lang F., Bauers K., Gerok W. Interactions between glutamine metabolism and cell-volume regulation in perfused rat liver. Eur J Biochem. 1990 Mar 30;188(3):689–695. doi: 10.1111/j.1432-1033.1990.tb15451.x. [DOI] [PubMed] [Google Scholar]
  5. Häussinger D., Lang F. Exposure of perfused liver to hypotonic conditions modifies cellular nitrogen metabolism. J Cell Biochem. 1990 Aug;43(4):355–361. doi: 10.1002/jcb.240430407. [DOI] [PubMed] [Google Scholar]
  6. Häussinger D., Stehle T., Lang F. Volume regulation in liver: further characterization by inhibitors and ionic substitutions. Hepatology. 1990 Feb;11(2):243–254. doi: 10.1002/hep.1840110214. [DOI] [PubMed] [Google Scholar]
  7. Kristensen L. O., Folke M. Volume-regulatory K+ efflux during concentrative uptake of alanine in isolated rat hepatocytes. Biochem J. 1984 Jul 1;221(1):265–268. doi: 10.1042/bj2210265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lang F., Stehle T., Häussinger D. Water, K+, H+, lactate and glucose fluxes during cell volume regulation in perfused rat liver. Pflugers Arch. 1989 Jan;413(3):209–216. doi: 10.1007/BF00583532. [DOI] [PubMed] [Google Scholar]
  9. Leverve X. M., Caro L. H., Plomp P. J., Meijer A. J. Control of proteolysis in perifused rat hepatocytes. FEBS Lett. 1987 Jul 27;219(2):455–458. doi: 10.1016/0014-5793(87)80271-5. [DOI] [PubMed] [Google Scholar]
  10. MacLennan P. A., Smith K., Weryk B., Watt P. W., Rennie M. J. Inhibition of protein breakdown by glutamine in perfused rat skeletal muscle. FEBS Lett. 1988 Sep 12;237(1-2):133–136. doi: 10.1016/0014-5793(88)80186-8. [DOI] [PubMed] [Google Scholar]
  11. Mortimore G. E., Lardeux B. R., Adams C. E. Regulation of microautophagy and basal protein turnover in rat liver. Effects of short-term starvation. J Biol Chem. 1988 Feb 15;263(5):2506–2512. [PubMed] [Google Scholar]
  12. Plomp P. J., Wolvetang E. J., Groen A. K., Meijer A. J., Gordon P. B., Seglen P. O. Energy dependence of autophagic protein degradation in isolated rat hepatocytes. Eur J Biochem. 1987 Apr 1;164(1):197–203. doi: 10.1111/j.1432-1033.1987.tb11011.x. [DOI] [PubMed] [Google Scholar]
  13. Pösö A. R., Schworer C. M., Mortimore G. E. Acceleration of proteolysis in perfused rat liver by deletion of glucogenic amino acids: regulatory role of glutamine. Biochem Biophys Res Commun. 1982 Aug 31;107(4):1433–1439. doi: 10.1016/s0006-291x(82)80159-9. [DOI] [PubMed] [Google Scholar]
  14. Schworer C. M., Mortimore G. E. Glucagon-induced autophagy and proteolysis in rat liver: mediation by selective deprivation of intracellular amino acids. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3169–3173. doi: 10.1073/pnas.76.7.3169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Seglen P. O., Gordon P. B. Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes. J Cell Biol. 1984 Aug;99(2):435–444. doi: 10.1083/jcb.99.2.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shinnick F. L., Harper A. E. Branched-chain amino acid oxidation by isolated rat tissue preparations. Biochim Biophys Acta. 1976 Jul 21;437(2):477–486. doi: 10.1016/0304-4165(76)90016-7. [DOI] [PubMed] [Google Scholar]
  17. Wettstein M., vom Dahl S., Lang F., Gerok W., Häussinger D. Cell volume regulatory responses of isolated perfused rat liver. The effect of amino acids. Biol Chem Hoppe Seyler. 1990 Jun;371(6):493–501. doi: 10.1515/bchm3.1990.371.1.493. [DOI] [PubMed] [Google Scholar]

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