Fig. 1.

RosA inhibits Mmp3, Mmp13, and Cox2 expression induced by IL-1β in articular chondrocytes.

(A, B, C, D) Chondrocytes were co-treated with IL-1β and RosA for 24 h Mmp3, Mmp13, and Cox2 expression were evaluated by RT-PCR (A), densitometry (B; Mmp3, C; Mmp13, and D; Cox2), western blot (E) and densitometry (F; Mmp3, G; Mmp13, H; Cox2) analysis. Collagenase activity (I) and PGE₂ production (J) were detected in chondrocytes cultured medium. Values are presented as the mean ± standard deviation. One-way analysis of variance followed by Bonferroni's test was used to determine significant differences. None significant (N.S), ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, #P < 0.05 compared to the control group. Original gel (Fig. S6) and blots (Fig. S7) can be seen in supplementary figures file.