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. 1991 Feb 15;274(Pt 1):27–33. doi: 10.1042/bj2740027

Multiple sodium-dependent nucleoside transport systems in bovine renal brush-border membrane vesicles.

T C Williams 1, S M Jarvis 1
PMCID: PMC1149915  PMID: 2001243

Abstract

Na(+)-dependent nucleoside transport was examined in bovine renal brush-border membrane vesicles. Two separate Na+/nucleoside cotransporters were shown to be present: (1) a system specific for purine nucleosides and uridine, designated as the N1 carrier, and (2) an Na(+)-dependent nucleoside transporter that accepts pyrimidine nucleosides, adenosine and analogues of adenosine, designated as the N2 system. Both systems exhibit a high affinity for nucleosides (apparent Km values approximately 10 microM), are insensitive to inhibition by facilitated-diffusion nucleoside transport inhibitors, are rheogenic and exhibit a high specificity for Na+. Na+ increases the affinity of the influx of guanosine and thymidine, nucleosides that serve as model permeants for the N1 and N2 nucleoside transporters respectively. The Na+/nucleoside coupling stoichiometry is consistent with 1:1 for both carriers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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