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. 1991 Apr 1;275(Pt 1):207–210. doi: 10.1042/bj2750207

Identification of a catalytically essential nucleophilic residue in sheep liver cytoplasmic aldehyde dehydrogenase.

T M Kitson 1, J P Hill 1, G G Midwinter 1
PMCID: PMC1150034  PMID: 2018476

Abstract

Sheep liver cytoplasmic aldehyde dehydrogenase was labelled by reaction with the substrate p-nitrophenyl di[14C]methylcarbamate. After tryptic digestion and peptide fractionation the labelled residue was identified as Cys-302. This is the first unequivocal identification of the essential enzymic nucleophile in the esterase activity of aldehyde dehydrogenase. By implication, Cys-302 is probably also the residue that is acylated by aldehyde substrates and the first residue that is modified by disulfiram.

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Selected References

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