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. 1991 Apr 1;275(Pt 1):277–280. doi: 10.1042/bj2750277

Cyclic AMP-evoked oscillations of intracellular [Ca2+] in guinea-pig hepatocytes.

T Capiod 1, J Noel 1, L Combettes 1, M Claret 1
PMCID: PMC1150046  PMID: 1850241

Abstract

The effects of the beta-adrenoceptor agonist isoprenaline and cyclic AMP (cAMP) on cytosolic free Ca2+ ([Ca2+]i) were studied in the single guinea-pig hepatocyte. In common with InsP3-dependent agonists such as noradrenaline or angiotensin II, isoprenaline (0.5-10 microM) and cAMP (50-100 mM, perfused into the cell via the patch-pipette), were able to generate fast and slow fluctuations of [Ca2+]i. Responses to isoprenaline and cAMP also were observed in the absence of external Ca2+. Isoprenaline-evoked [Ca2+]i rises were not blocked by the intracellular perfusion of heparin, suggesting that these fluctuations are independent of the binding of InsP3 to its receptor.

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Selected References

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