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. 1991 May 1;275(Pt 3):785–788. doi: 10.1042/bj2750785

Solution structures of calcitonin-gene-related-peptide analogues of calcitonin-gene-related peptide and amylin.

J A Hubbard 1, S R Martin 1, L C Chaplin 1, C Bose 1, S M Kelly 1, N C Price 1
PMCID: PMC1150122  PMID: 2039456

Abstract

Near-u.v. and far-u.v. c.d. spectra of human alpha-calcitonin-gene-related peptide (h alpha CGRP), analogues and fragments of CGRP and amylin were recorded in aqueous solution and in trifluoroethanol (TFE)/water mixtures. All peptides contained significant amounts of alpha-helix in aqueous solution, and this amount increased on adding TFE. The helical content was unaffected by pH and salt. However, amylin contained much less helix than CGRP and the c.d. spectrum was more temperature-sensitive. A band in the near-u.v. c.d. spectrum of CGRP (but not present in the spectrum of amylin) was attributed to the disulphide bond in CGRP. The intensity of this band was pH-dependent and titrated with a pKa of 6.5, suggesting the involvement of histidine ionization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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