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. 1991 May 1;275(Pt 3):789–791. doi: 10.1042/bj2750789

Oxidation of thiol in the vimentin cytoskeleton.

K R Rogers 1, C J Morris 1, D R Blake 1
PMCID: PMC1150123  PMID: 2039457

Abstract

Sublethal doses of H2O2, which induces oxidative stress, cause substantial alteration to the vimentin cytoskeleton in various cell types. We have used a thiol-blot assay to assess thiol status in individual proteins from cell extracts. Vimentin thiol is oxidized in preference to other cytoskeleton proteins. Immunoblot analysis also demonstrated a loss of reactivity to an anti-vimentin monoclonal antibody under non-reducing conditions, possibly due to thiol-group oxidation. During induced oxidative stress a number of proteins become associated with the cytoskeleton extracts.

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Selected References

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