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. 1991 Mar 1;274(Pt 2):375–379. doi: 10.1042/bj2740375

An acetylation method for the quantification of membrane lipids, including phospholipids, polyphosphoinositides and cholesterol.

J M Stein 1, G A Smith 1, J P Luzio 1
PMCID: PMC1150147  PMID: 1848754

Abstract

A method for the quantification of membrane lipids by acetylation with [3H]acetic anhydride is described. A standard lipid, labelled with 14C or 32P, is added to the sample and is simultaneously acetylated. The 3H/14C or 3H/32P ratios obtained in the acetylated lipids are proportional to the initial lipid concentration. The method has been used to quantify cholesterol, phosphatidylethanolamine, phosphatidylinositol, sphingomyelin, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in amounts between 2 and 10 nmol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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