Abstract
A method is presented for fitting the pairs of values product formed-time taken from progress curves to the integrated rate equation. The procedure is applied to the estimation of the kinetic parameters of the adenosine deaminase system. Simulation studies demonstrate the capabilities of this strategy. A copy of the FORTRAN77 program used can be obtained from the authors by request.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Canela E. I. A free derivative program for non-linear regression analysis of enzyme kinetics to be used on small computers. Int J Biomed Comput. 1984 Mar-Apr;15(2):121–130. doi: 10.1016/0020-7101(84)90024-2. [DOI] [PubMed] [Google Scholar]
- Canela E. I., Franco R. Enzyme kinetic studies from progress curves. Biochem J. 1986 Jan 15;233(2):599–605. doi: 10.1042/bj2330599. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duggleby R. G., Nash J. C. A single-parameter family of adjustments for fitting enzyme kinetic models to progress-curve data. Biochem J. 1989 Jan 1;257(1):57–64. doi: 10.1042/bj2570057. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Franco R., Canela E. I. A program for the numerical integration of enzyme kinetic equations using small computers. Int J Biomed Comput. 1984 Nov-Dec;15(6):419–432. doi: 10.1016/0020-7101(84)90013-8. [DOI] [PubMed] [Google Scholar]
- Selwyn M. J. A simple test for inactivation of an enzyme during assay. Biochim Biophys Acta. 1965 Jul 29;105(1):193–195. doi: 10.1016/s0926-6593(65)80190-4. [DOI] [PubMed] [Google Scholar]