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. 1991 Mar 1;274(Pt 2):607–609. doi: 10.1042/bj2740607

c-myc mRNA in cytoskeletal-bound polysomes in fibroblasts.

J E Hesketh 1, G P Campbell 1, P F Whitelaw 1
PMCID: PMC1150182  PMID: 2006923

Abstract

3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, 130 mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in order to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta 2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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