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. 1991 Mar 1;274(Pt 2):615–617. doi: 10.1042/bj2740615

Relative rates of biosynthesis of collagen type I, type V and type VI in calf cornea.

P Kern 1, M Menasche 1, L Robert 1
PMCID: PMC1150184  PMID: 2006924

Abstract

The biosynthesis of type I, type V and type VI collagens was studied by incubation of calf corneas in vitro with [3H]proline as a marker. Pepsin-solubilized collagen types were isolated by salt fractionation and quantified by SDS/PAGE. Expressed as proportions of the total hydroxyproline solubilized, corneal stroma comprised 75% type I, 8% type V and 17% type VI collagen. The rates of [3H]proline incorporation, linear up to 24 h for each collagen type, were highest for type VI collagen and lowest for type I collagen. From pulse-chase experiments, the calculated apparent half-lives for types I, V and VI collagens were 36 h, 10 h and 6 h respectively.

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Selected References

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