Table 1.
Polar lipids identified in P. vulgaris varieties through LC-MS analysis in negative ion mode.
| N° | Compound | Rt (min) | Molecular Formula |
[M − H]− | ppm | MS/MS |
|---|---|---|---|---|---|---|
| 1 | 9,10,13-TriHODE | 13.05 | C18H32O5 | 327.2163 | 0.02 | 211.13/291.20/309.21/269.17/183.14/141.18 |
| 2 | 9,12,13-TriHOME (10) | 13.72 | C18H34O5 | 329.2320 | −0.12 | 229.14/311.22/293.21/211.13/171.10 |
| 3 | 10,16-Dihydroxyhexadecanoic acid | 14.75 | C16H32O4 | 287.2217 | 0.29 | 269.21/141.18/189.98/109.03 |
| 4 | 11-methyl dodecadienoic acid | 17.05 | C13H22O2 | 209.1538 | −0.97 | 165.16515 |
| 5 | L-PI (18:3) | 17.51 | C27H47O12P | 593.2719 | −0.38 | 315.04/241.01/277.21 |
| 6 | 9,10-DiHODE | 18.37 | C18H32O4 | 311.2222 | 1.65 | 201.11/275.20/293.21/171.10 |
| 7 | L-PE (18:3) | 18.50 | C23H42NO7P | 474.2620 | 0.36 | 277.21/214.05/196.04 |
| 8 | 15,16-DiHODE | 18.52 | C18H32O4 | 311.2221 | 0.55 | 223.17/235.17/275.20/293.21 |
| 9 | PE(18:1(9Z)/0:0) | 19.12 | C23H46NO7P | 478.2928 | 0.01 | 281.24/214.05/196.04 |
| 10 | 9,11-Linoleic acid | 19.78 | C18H32O2 | 279.2323 | 1.69 | 183.70/112.9 |
| 11 | L-PE (18:2) | 19.78 | C23H44NO7P | 476.2776 | 0.36 | 279.23/214.05/196.04 |
| 12 | OKHdiA-PE | 20.15 | C30H52NO11P | 632.3192 | −0.29 | 279.23/255.23/112.98/ |
| 13 | PE(16:0/0:0) | 20.19 | C21H44NO7P | 452.2769 | −0.47 | 255.23/214.05/196.04 |
| 14 | PI (16:0_18:3) | 20.40 | C43H77O13P | 831.5008 | −1.14 | 770.57/553.28/391.22/277.22/255.23/241.01/297.04/ |
| 15 | L-PE (16:0) | 20.63 | C21H44NO7P | 452.2776 | 0.31 | 255.23/214.05/196.04 |
| 16 | Palmitic acid | 20.63 | C16H32O2 | 255.2322 | 1.61 | 214.07/187.06/145.02/112.98 |
| 17 | PE(18:0/0:0) | 20.70 | C23H48NO7P | 480.3084 | 0.13 | 255.23/224.07/214.05/196.04/153.00 |
| 18 | PE(16:0/18:3(9Z,12Z,15Z)) | 20.99 | C39H72NO8P | 712.4914 | 0.39 | 277.22/255.23/452.28/214.05/196.04 |
| 19 | PHHdiA-PE | 21.13 | C28H52NO11P | 608.3195 | 0.15 | 255.23/313.61/401.61/112.98 |
| 20 | (6Z)-Octadecenoicacid | 21.47 | C18H34O2 | 281.2479 | 1.61 | 106.04/171.07/212.09 |
| 21 | L-PE (18:1) | 21.47 | C23H46NO7P | 478.2930 | 0.51 | 281.24/214.05/196.04 |
| 22 | (9Z)-(13S)-12_13-Epoxyoctadeca-9,11-dienoic acid | 22.14 | C18H30O3 | 293.2116 | −1.25 | 236.10551; 221.15480 |
| 23 | PE (16:0, 18:2) | 22.84 | C39H74NO8P | 714.5062 | −0.75 | 452.27/279.23/ 255.23/214.05/196.04 |
Phosphatidylethanolamine (PE); lyso-forms of phosphatidylethanolamine (L-PE); phosphatidylinositol (PI); lyso-forms of phosphatidylinositol (L-PI); in bold, diagnostic product ions. The compounds were identified using the “Compound Discoverer software version 3.3.3.200” platform and literature data, such as references [45,46,47,48].