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. 2024 Oct 21;13(20):1744. doi: 10.3390/cells13201744

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Global features of mRNA m⁶A methylation in LW and NX pig muscles. (A) Workflow for detection of m⁶A modifications in NX and LW pig muscle. m⁶A-containing mRNA fragments were purified via immunoprecipitation (IP). (B) The average number of m⁶A peaks in each group. (C) Density of m⁶A peaks along transcripts. (D) The enriched consensus motif of m⁶As in LW and NX pig muscles. (E) The distribution of m⁶A enriched regions on the chromosomes of NX−1 pig. GO (F) and KEGG (G) enrichment analysis in 2035 genes with m⁶A modification in NX−1 pig muscle samples.