Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1991 Jun 1;276(Pt 2):559–561. doi: 10.1042/bj2760559

Effects of changes in cell volume on the rates of glutamine and alanine release from rat skeletal muscle in vitro.

M Parry-Billings 1, S J Bevan 1, E Opara 1, E A Newsholme 1
PMCID: PMC1151128  PMID: 2049080

Abstract

The effect of changes in cell volume on the rates of release of glutamine and alanine from muscle and on the concentrations of these amino acids in muscle were investigated by using an isolated preparation of rat skeletal muscle incubated in the presence of hypo- and hyper-osmotic media. Changes in cell volume were associated with changes in the rates of release of glutamine and alanine from muscle: incubation in hypo-osmotic medium decreased the rates of release of glutamine and alanine, and incubation in hyperosmotic medium increased these rates. These changes were rapidly reversed by a change in osmoticity of the medium. Despite marked changes in cell volume, the concentrations of these amino acids in muscle were maintained. It is suggested that cell volume may play a role in the regulation of amino acid metabolism in skeletal muscle.

Full text

PDF
559

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmed A., Taylor P. M., Rennie M. J. Characteristics of glutamine transport in sarcolemmal vesicles from rat skeletal muscle. Am J Physiol. 1990 Aug;259(2 Pt 1):E284–E291. doi: 10.1152/ajpendo.1990.259.2.E284. [DOI] [PubMed] [Google Scholar]
  2. Ardawi M. S. Skeletal muscle glutamine production in thermally injured rats. Clin Sci (Lond) 1988 Feb;74(2):165–172. doi: 10.1042/cs0740165. [DOI] [PubMed] [Google Scholar]
  3. Baquet A., Hue L., Meijer A. J., van Woerkom G. M., Plomp P. J. Swelling of rat hepatocytes stimulates glycogen synthesis. J Biol Chem. 1990 Jan 15;265(2):955–959. [PubMed] [Google Scholar]
  4. Hundal H. S., Rennie M. J., Watt P. W. Characteristics of L-glutamine transport in perfused rat skeletal muscle. J Physiol. 1987 Dec;393:283–305. doi: 10.1113/jphysiol.1987.sp016824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Häussinger D., Lang F., Bauers K., Gerok W. Interactions between glutamine metabolism and cell-volume regulation in perfused rat liver. Eur J Biochem. 1990 Mar 30;188(3):689–695. doi: 10.1111/j.1432-1033.1990.tb15451.x. [DOI] [PubMed] [Google Scholar]
  6. Karl I. E., Garber A. J., Kipnis D. M. Alanine and glutamine synthesis and release from skeletal muscle. III. Dietary and hormonal regulation. J Biol Chem. 1976 Feb 10;251(3):844–850. [PubMed] [Google Scholar]
  7. King P. A., Goldstein L., Newsholme E. A. Glutamine synthetase activity of muscle in acidosis. Biochem J. 1983 Nov 15;216(2):523–525. doi: 10.1042/bj2160523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Newsholme E. A., Crabtree B., Ardawi M. S. Glutamine metabolism in lymphocytes: its biochemical, physiological and clinical importance. Q J Exp Physiol. 1985 Oct;70(4):473–489. doi: 10.1113/expphysiol.1985.sp002935. [DOI] [PubMed] [Google Scholar]
  9. Parry-Billings M., Leighton B., Dimitriadis G., de Vasconcelos P. R., Newsholme E. A. Skeletal muscle glutamine metabolism during sepsis in the rat. Int J Biochem. 1989;21(4):419–423. doi: 10.1016/0020-711x(89)90366-2. [DOI] [PubMed] [Google Scholar]
  10. Peeze Binkhorst F. M., Slaaf D. W., Kuipers H., Tangelder G. J., Reneman R. S. Exercise-induced swelling of rat soleus muscle: its relationship with intramuscular pressure. J Appl Physiol (1985) 1990 Jul;69(1):67–73. doi: 10.1152/jappl.1990.69.1.67. [DOI] [PubMed] [Google Scholar]
  11. Rennie M. J., Hundal H. S., Babij P., MacLennan P., Taylor P. M., Watt P. W., Jepson M. M., Millward D. J. Characteristics of a glutamine carrier in skeletal muscle have important consequences for nitrogen loss in injury, infection, and chronic disease. Lancet. 1986 Nov 1;2(8514):1008–1012. doi: 10.1016/s0140-6736(86)92617-6. [DOI] [PubMed] [Google Scholar]
  12. Schröck H., Cha C. J., Goldstein L. Glutamine release from hindlimb and uptake by kidney in the acutely acidotic rat. Biochem J. 1980 May 15;188(2):557–560. doi: 10.1042/bj1880557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Watford M. A 'swell' way to regulate metabolism. Trends Biochem Sci. 1990 Sep;15(9):329–330. doi: 10.1016/0968-0004(90)90065-j. [DOI] [PubMed] [Google Scholar]
  14. Windmueller H. G., Spaeth A. E. Uptake and metabolism of plasma glutamine by the small intestine. J Biol Chem. 1974 Aug 25;249(16):5070–5079. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES