Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1991 Jul 15;277(Pt 2):341–344. doi: 10.1042/bj2770341

Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation.

H O Hansen 1, P H Andreasen 1, S Mandrup 1, K Kristiansen 1, J Knudsen 1
PMCID: PMC1151239  PMID: 1859362

Abstract

The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.

Full text

PDF
341

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Berge R. K., Aarsland A. Correlation between the cellular level of long-chain acyl-CoA, peroxisomal beta-oxidation, and palmitoyl-CoA hydrolase activity in rat liver. Are the two enzyme systems regulated by a substrate-induced mechanism? Biochim Biophys Acta. 1985 Nov 14;837(2):141–151. doi: 10.1016/0005-2760(85)90237-1. [DOI] [PubMed] [Google Scholar]
  3. Bernlohr D. A., Angus C. W., Lane M. D., Bolanowski M. A., Kelly T. J., Jr Expression of specific mRNAs during adipose differentiation: identification of an mRNA encoding a homologue of myelin P2 protein. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5468–5472. doi: 10.1073/pnas.81.17.5468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brunk C. F., Jones K. C., James T. W. Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem. 1979 Jan 15;92(2):497–500. doi: 10.1016/0003-2697(79)90690-0. [DOI] [PubMed] [Google Scholar]
  5. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  6. Gray P. W. Molecular biology of diazepam binding inhibitor. Neuropharmacology. 1987 Jul;26(7B):863–865. doi: 10.1016/0028-3908(87)90063-3. [DOI] [PubMed] [Google Scholar]
  7. Knudsen J., Højrup P., Hansen H. O., Hansen H. F., Roepstorff P. Acyl-CoA-binding protein in the rat. Purification, binding characteristics, tissue concentrations and amino acid sequence. Biochem J. 1989 Sep 1;262(2):513–519. doi: 10.1042/bj2620513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knudsen J., Nielsen M. Diazepam-binding inhibitor: a neuropeptide and/or an acyl-CoA ester binding protein? Biochem J. 1990 Feb 1;265(3):927–929. doi: 10.1042/bj2650927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mackall J. C., Student A. K., Polakis S. E., Lane M. D. Induction of lipogenesis during differentiation in a "preadipocyte" cell line. J Biol Chem. 1976 Oct 25;251(20):6462–6464. [PubMed] [Google Scholar]
  10. Maden B. E., Dent C. L., Farrell T. E., Garde J., McCallum F. S., Wakeman J. A. Clones of human ribosomal DNA containing the complete 18 S-rRNA and 28 S-rRNA genes. Characterization, a detailed map of the human ribosomal transcription unit and diversity among clones. Biochem J. 1987 Sep 1;246(2):519–527. doi: 10.1042/bj2460519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mandrup S., Højrup P., Kristiansen K., Knudsen J. Gene synthesis, expression in Escherichia coli, purification and characterization of the recombinant bovine acyl-CoA-binding protein. Biochem J. 1991 Jun 15;276(Pt 3):817–823. doi: 10.1042/bj2760817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marquardt H., Todaro G. J., Shoyab M. Complete amino acid sequences of bovine and human endozepines. Homology with rat diazepam binding inhibitor. J Biol Chem. 1986 Jul 25;261(21):9727–9731. [PubMed] [Google Scholar]
  13. Mikkelsen J., Højrup P., Nielsen P. F., Roepstorff P., Knudsen J. Amino acid sequence of acyl-CoA-binding protein from cow liver. Biochem J. 1987 Aug 1;245(3):857–861. doi: 10.1042/bj2450857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mikkelsen J., Knudsen J. Acyl-CoA-binding protein from cow. Binding characteristics and cellular and tissue distribution. Biochem J. 1987 Dec 15;248(3):709–714. doi: 10.1042/bj2480709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mocchetti I., Einstein R., Brosius J. Putative diazepam binding inhibitor peptide: cDNA clones from rat. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7221–7225. doi: 10.1073/pnas.83.19.7221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mogensen I. B., Schulenberg H., Hansen H. O., Spener F., Knudsen J. A novel acyl-CoA-binding protein from bovine liver. Effect on fatty acid synthesis. Biochem J. 1987 Jan 1;241(1):189–192. doi: 10.1042/bj2410189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ogiwara H., Tanabe T., Nikawa J., Numa S. Inhibition of rat-liver acetyl-coenzyme-A carboxylase by palmitoyl-coenzyme A. Formation of equimolar enzyme-inhibitor complex. Eur J Biochem. 1978 Aug 15;89(1):33–41. doi: 10.1111/j.1432-1033.1978.tb20893.x. [DOI] [PubMed] [Google Scholar]
  18. Palmiter R. D. Ovalbumin messenger ribonucleic acid translation. Comparable rates of polypeptide initiation and elongation on ovalbumin and globin messenger ribonucleic acid in a rabbit reticulocyte lysate. J Biol Chem. 1973 Mar 25;248(6):2095–2106. [PubMed] [Google Scholar]
  19. Pawlak J., Smith S. Evaluation of thioesterase II as a serum marker for rat mammary cancer. Cancer Res. 1986 Sep;46(9):4712–4719. [PubMed] [Google Scholar]
  20. Pfanner N., Orci L., Glick B. S., Amherdt M., Arden S. R., Malhotra V., Rothman J. E. Fatty acyl-coenzyme A is required for budding of transport vesicles from Golgi cisternae. Cell. 1989 Oct 6;59(1):95–102. doi: 10.1016/0092-8674(89)90872-6. [DOI] [PubMed] [Google Scholar]
  21. Rasmussen J. T., Börchers T., Knudsen J. Comparison of the binding affinities of acyl-CoA-binding protein and fatty-acid-binding protein for long-chain acyl-CoA esters. Biochem J. 1990 Feb 1;265(3):849–855. doi: 10.1042/bj2650849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Reed B. C., Lane M. D. Insulin receptor synthesis and turnover in differentiating 3T3-L1 preadipocytes. Proc Natl Acad Sci U S A. 1980 Jan;77(1):285–289. doi: 10.1073/pnas.77.1.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sacchettini J. C., Said B., Schulz H., Gordon J. I. Rat heart fatty acid-binding protein is highly homologous to the murine adipocyte 422 protein and the P2 protein of peripheral nerve myelin. J Biol Chem. 1986 Jun 25;261(18):8218–8223. [PubMed] [Google Scholar]
  24. Schacterle G. R., Pollack R. L. A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal Biochem. 1973 Feb;51(2):654–655. doi: 10.1016/0003-2697(73)90523-x. [DOI] [PubMed] [Google Scholar]
  25. Shoyab M., Gentry L. E., Marquardt H., Todaro G. J. Isolation and characterization of a putative endogenous benzodiazepineoid (endozepine) from bovine and human brain. J Biol Chem. 1986 Sep 15;261(26):11968–11973. [PubMed] [Google Scholar]
  26. Sumper M., Träuble H. Membranes as acceptors for palmitoyl CoA in fatty acid biosynthesis. FEBS Lett. 1973 Feb 15;30(1):29–34. doi: 10.1016/0014-5793(73)80612-x. [DOI] [PubMed] [Google Scholar]
  27. Webb N. R., Rose T. M., Malik N., Marquardt H., Shoyab M., Todaro G. J., Lee D. C. Bovine and human cDNA sequences encoding a putative benzodiazepine receptor ligand. DNA. 1987 Feb;6(1):71–79. doi: 10.1089/dna.1987.6.71. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES