Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1991 Jul 15;277(Pt 2):561–563. doi: 10.1042/bj2770561

Expression of a human monoclonal anti-(rhesus D) Fab fragment in Escherichia coli with the use of bacteriophage lambda vectors.

R A Williamson 1, M A Persson 1, D R Burton 1
PMCID: PMC1151271  PMID: 1830475

Abstract

A human anti-(rhesus D) antibody (IgG1 lambda) Fab fragment was cloned from an Epstein-Barr-virus-transformed cell line and expressed in Escherichia coli with the use of bacteriophage lambda vectors. The cloned protein is active in binding to human erythrocytes and permits the development of a recombinant reagent for the prevention of haemolytic disease of the newborn. The method offers a rapid and effective means of rescuing human Fabs from potentially unstable cell lines secreting human antibodies.

Full text

PDF
561

Images in this article

562Fig. 1.
on p.562

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Better M., Horwitz A. H. Expression of engineered antibodies and antibody fragments in microorganisms. Methods Enzymol. 1989;178:476–496. doi: 10.1016/0076-6879(89)78035-6. [DOI] [PubMed] [Google Scholar]
  2. Casali P., Notkins A. L. Probing the human B-cell repertoire with EBV: polyreactive antibodies and CD5+ B lymphocytes. Annu Rev Immunol. 1989;7:513–535. doi: 10.1146/annurev.iy.07.040189.002501. [DOI] [PubMed] [Google Scholar]
  3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  4. Crawford D. H., Barlow M. J., Harrison J. F., Winger L., Huehns E. R. Production of human monoclonal antibody to rhesus D antigen. Lancet. 1983 Feb 19;1(8321):386–388. doi: 10.1016/s0140-6736(83)91502-7. [DOI] [PubMed] [Google Scholar]
  5. Doyle A., Jones T. J., Bidwell J. L., Bradley B. A. In vitro development of human monoclonal antibody-secreting plasmacytomas. Hum Immunol. 1985 Jul;13(3):199–209. doi: 10.1016/0198-8859(85)90012-6. [DOI] [PubMed] [Google Scholar]
  6. Foung S. K., Blunt J. A., Wu P. S., Ahearn P., Winn L. C., Engleman E. G., Grumet F. C. Human monoclonal antibodies to Rh0(D). Vox Sang. 1987;53(1):44–47. doi: 10.1111/j.1423-0410.1987.tb04912.x. [DOI] [PubMed] [Google Scholar]
  7. Goossens D., Champomier F., Rouger P., Salmon C. Human monoclonal antibodies against blood group antigens. Preparation of a series of stable EBV immortalized B clones producing high levels of antibody of different isotypes and specificities. J Immunol Methods. 1987 Aug 3;101(2):193–200. doi: 10.1016/0022-1759(87)90150-5. [DOI] [PubMed] [Google Scholar]
  8. Huse W. D., Sastry L., Iverson S. A., Kang A. S., Alting-Mees M., Burton D. R., Benkovic S. J., Lerner R. A. Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Science. 1989 Dec 8;246(4935):1275–1281. doi: 10.1126/science.2531466. [DOI] [PubMed] [Google Scholar]
  9. Melamed M. D., Gordon J., Ley S. J., Edgar D., Hughes-Jones N. C. Senescence of a human lymphoblastoid clone producing anti-Rhesus(D). Eur J Immunol. 1985 Jul;15(7):742–746. doi: 10.1002/eji.1830150720. [DOI] [PubMed] [Google Scholar]
  10. Paire J., Monestier M., Rigal D., Martel F., Desgranges C. Establishment of human cell lines producing anti-D monoclonal antibodies: identification of Rhesus D antigen. Immunol Lett. 1986 Sep;13(3):137–141. doi: 10.1016/0165-2478(86)90046-5. [DOI] [PubMed] [Google Scholar]
  11. Robinson W. E., Jr, Kawamura T., Gorny M. K., Lake D., Xu J. Y., Matsumoto Y., Sugano T., Masuho Y., Mitchell W. M., Hersh E. Human monoclonal antibodies to the human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 enhance HIV-1 infection in vitro. Proc Natl Acad Sci U S A. 1990 Apr;87(8):3185–3189. doi: 10.1073/pnas.87.8.3185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Urbaniak S. J. Rh(D) haemolytic disease of the newborn: the changing scene. Br Med J (Clin Res Ed) 1985 Jul 6;291(6487):4–6. doi: 10.1136/bmj.291.6487.4. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES