Abstract
Tartrate-resistant acid phosphatases have been isolated from a number of sources. These enzymes consist of one subunit (Mr 30,000-40,000) or two dissimilar subunits (Mr 15,000-20,000). Previously we isolated the enzyme from human osteoclastomas, as a two-subunit protein. By Northern blotting and hybridization with radiolabelled oligonucleotides corresponding to the N-terminal sequences of the two subunits, we demonstrate here that the enzyme is transcribed as one mRNA which is translated in vitro to produce a single polypeptide of approx. Mr 33,000. Transcription as a single mRNA species is also the case in other tissues. These results suggest that the osteoclastoma enzyme undergoes post-translational modification in the form of cleavage of a single peptide bond to give a disulphide-bonded two-subunit protein.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allen S. H., Nuttleman P. R., Ketcham C. M., Roberts R. M. Purification and characterization of human bone tartrate-resistant acid phosphatase. J Bone Miner Res. 1989 Feb;4(1):47–55. doi: 10.1002/jbmr.5650040108. [DOI] [PubMed] [Google Scholar]
- Anderson R. E., Woodbury D. M., Jee W. S. Humoral and ionic regulation of osteoclast acidity. Calcif Tissue Int. 1986 Oct;39(4):252–258. doi: 10.1007/BF02555214. [DOI] [PubMed] [Google Scholar]
- Anderson T. R., Toverud S. U. Purification and characterization of purple acid phosphatase from developing rat bone. Arch Biochem Biophys. 1986 May 15;247(1):131–139. doi: 10.1016/0003-9861(86)90541-2. [DOI] [PubMed] [Google Scholar]
- Andersson G., Ek-Rylander B., Hammarström L. Purification and characterization of a vanadate-sensitive nucleotide tri- and diphosphatase with acid pH optimum from rat bone. Arch Biochem Biophys. 1984 Feb 1;228(2):431–438. doi: 10.1016/0003-9861(84)90007-9. [DOI] [PubMed] [Google Scholar]
- Buhi W. C., Ducsay C. A., Bazer F. W., Roberts R. M. Iron transfer between the purple phosphatase uteroferrin and transferrin and its possible role in iron metabolism of the fetal pig. J Biol Chem. 1982 Feb 25;257(4):1712–1723. [PubMed] [Google Scholar]
- Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
- Davis J. C., Lin S. S., Averill B. A. Kinetics and optical spectroscopic studies on the purple acid phosphatase from beef spleen. Biochemistry. 1981 Jul 7;20(14):4062–4067. doi: 10.1021/bi00517a018. [DOI] [PubMed] [Google Scholar]
- Hayman A. R., Warburton M. J., Pringle J. A., Coles B., Chambers T. J. Purification and characterization of a tartrate-resistant acid phosphatase from human osteoclastomas. Biochem J. 1989 Jul 15;261(2):601–609. doi: 10.1042/bj2610601. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kato T., Hara A., Nakayama T., Sawada H., Hamatake M., Matsumoto Y. Purification and characterization of purple acid phosphatase from rat bone. Comp Biochem Physiol B. 1986;83(4):813–817. doi: 10.1016/0305-0491(86)90152-5. [DOI] [PubMed] [Google Scholar]
- Ketcham C. M., Baumbach G. A., Bazer F. W., Roberts R. M. The type 5, acid phosphatase from spleen of humans with hairy cell leukemia. Purification, properties, immunological characterization, and comparison with porcine uteroferrin. J Biol Chem. 1985 May 10;260(9):5768–5776. [PubMed] [Google Scholar]
- Ketcham C. M., Roberts R. M., Simmen R. C., Nick H. S. Molecular cloning of the type 5, iron-containing, tartrate-resistant acid phosphatase from human placenta. J Biol Chem. 1989 Jan 5;264(1):557–563. [PubMed] [Google Scholar]
- Lau K. H., Freeman T. K., Baylink D. J. Purification and characterization of an acid phosphatase that displays phosphotyrosyl-protein phosphatase activity from bovine cortical bone matrix. J Biol Chem. 1987 Jan 25;262(3):1389–1397. [PubMed] [Google Scholar]
- Lau K. H., Onishi T., Wergedal J. E., Singer F. R., Baylink D. J. Characterization and assay of tartrate-resistant acid phosphatase activity in serum: potential use to assess bone resorption. Clin Chem. 1987 Apr;33(4):458–462. [PubMed] [Google Scholar]
- Lord D. K., Cross N. C., Bevilacqua M. A., Rider S. H., Gorman P. A., Groves A. V., Moss D. W., Sheer D., Cox T. M. Type 5 acid phosphatase. Sequence, expression and chromosomal localization of a differentiation-associated protein of the human macrophage. Eur J Biochem. 1990 Apr 30;189(2):287–293. doi: 10.1111/j.1432-1033.1990.tb15488.x. [DOI] [PubMed] [Google Scholar]
- Reinholt F. P., Widholm S. M., Ek-Rylander B., Andersson G. Ultrastructural localization of a tartrate-resistant acid ATPase in bone. J Bone Miner Res. 1990 Oct;5(10):1055–1061. doi: 10.1002/jbmr.5650051009. [DOI] [PubMed] [Google Scholar]
- Robinson D. B., Glew R. H. A tartrate-resistant acid phosphatase from Gaucher spleen. Purification and properties. J Biol Chem. 1980 Jun 25;255(12):5864–5870. [PubMed] [Google Scholar]
- Robinson D. B., Glew R. H. Acid phosphatase in Gaucher's disease. Clin Chem. 1980 Mar;26(3):371–382. [PubMed] [Google Scholar]
- Schindelmeiser J., Münstermann D., Witzel H. Histochemical investigations on the localization of the purple acid phosphatase in the bovine spleen. Histochemistry. 1987;87(1):13–19. doi: 10.1007/BF00518719. [DOI] [PubMed] [Google Scholar]
- Schlosnagle D. C., Bazer F. W., Tsibris J. C., Roberts R. M. An iron-containing phosphatase induced by progesterone in the uterine fluids of pigs. J Biol Chem. 1974 Dec 10;249(23):7574–7579. [PubMed] [Google Scholar]
- Stepan J. J., Lau K. H., Mohan S., Singer F. R., Baylink D. J. Purification and N-terminal amino acid sequence of the tartrate-resistant acid phosphatase from human osteoclastoma: evidence for a single structure. Biochem Biophys Res Commun. 1990 Apr 30;168(2):792–800. doi: 10.1016/0006-291x(90)92391-c. [DOI] [PubMed] [Google Scholar]
- Stepán J. J., Lau K. H., Mohan S., Kraenzlin M., Baylink D. J. Purification and N-terminal sequence of two tartrate-resistant acid phosphatases type-5 from the hairy cell leukemia spleen. Biochem Biophys Res Commun. 1989 Dec 29;165(3):1027–1034. doi: 10.1016/0006-291x(89)92705-8. [DOI] [PubMed] [Google Scholar]
- Waheed A., Laidler P. M., Wo Y. Y., Van Etten R. L. Purification and physicochemical characterization of a human placental acid phosphatase possessing phosphotyrosyl protein phosphatase activity. Biochemistry. 1988 Jun 14;27(12):4265–4273. doi: 10.1021/bi00412a010. [DOI] [PubMed] [Google Scholar]
- Yam L. T., Li C. Y., Finkel H. E. Leukemic reticuloendotheliosis. The role of tartrate-resistant acid phosphatase in diagnosis and splenectomy in treatment. Arch Intern Med. 1972 Aug;130(2):248–256. doi: 10.1001/archinte.130.2.248. [DOI] [PubMed] [Google Scholar]