Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1991 Sep 1;278(Pt 2):441–445. doi: 10.1042/bj2780441

Mammalian cartilage synthesizes both proteoglycan and non-proteoglycan forms of type IX collagen.

S Ayad 1, A Marriott 1, V H Brierley 1, M E Grant 1
PMCID: PMC1151363  PMID: 1898337

Abstract

Bovine epiphysial cartilage synthesizes both proteoglycan (PG) and non-PG forms of type IX collagen in a ratio of approx. 2:1. The PG form with its attached glycosaminoglycan on the alpha 2(IX) chain is the major form in the medium, whereas both forms are found in the tissue. The results are discussed with regard to cartilage matrix organization.

Full text

PDF
441

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ayad S., Abedin M. Z., Grundy S. M., Weiss J. B. Isolation and characterisation of an unusual collagen from hyaline cartilage and intervertebral disc. FEBS Lett. 1981 Jan 26;123(2):195–199. doi: 10.1016/0014-5793(81)80286-4. [DOI] [PubMed] [Google Scholar]
  2. Ayad S., Marriott A., Morgan K., Grant M. E. Bovine cartilage types VI and IX collagens. Characterization of their forms in vivo. Biochem J. 1989 Sep 15;262(3):753–761. doi: 10.1042/bj2620753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bruckner P., Mendler M., Steinmann B., Huber S., Winterhalter K. H. The structure of human collagen type IX and its organization in fetal and infant cartilage fibrils. J Biol Chem. 1988 Nov 15;263(32):16911–16917. [PubMed] [Google Scholar]
  4. Cooper A. R., Kurkinen M., Taylor A., Hogan B. L. Studies on the biosynthesis of laminin by murine parietal endoderm cells. Eur J Biochem. 1981 Sep;119(1):189–197. doi: 10.1111/j.1432-1033.1981.tb05593.x. [DOI] [PubMed] [Google Scholar]
  5. Eyre D. R., Apon S., Wu J. J., Ericsson L. H., Walsh K. A. Collagen type IX: evidence for covalent linkages to type II collagen in cartilage. FEBS Lett. 1987 Aug 17;220(2):337–341. doi: 10.1016/0014-5793(87)80842-6. [DOI] [PubMed] [Google Scholar]
  6. Gisslow M. T., McBride B. C. A rapid sensitive collagenase assay. Anal Biochem. 1975 Sep;68(1):70–78. doi: 10.1016/0003-2697(75)90680-6. [DOI] [PubMed] [Google Scholar]
  7. Huber S., Winterhalter K. H., Vaughan L. Isolation and sequence analysis of the glycosaminoglycan attachment site of type IX collagen. J Biol Chem. 1988 Jan 15;263(2):752–756. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  10. Mendler M., Eich-Bender S. G., Vaughan L., Winterhalter K. H., Bruckner P. Cartilage contains mixed fibrils of collagen types II, IX, and XI. J Cell Biol. 1989 Jan;108(1):191–197. doi: 10.1083/jcb.108.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Noro A., Kimata K., Oike Y., Shinomura T., Maeda N., Yano S., Takahashi N., Suzuki S. Isolation and characterization of a third proteoglycan (PG-Lt) from chick embryo cartilage which contains disulfide-bonded collagenous polypeptide. J Biol Chem. 1983 Aug 10;258(15):9323–9331. [PubMed] [Google Scholar]
  12. Oike Y., Kimata K., Shinomura T., Nakazawa K., Suzuki S. Structural analysis of chick-embryo cartilage proteoglycan by selective degradation with chondroitin lyases (chondroitinases) and endo-beta-D-galactosidase (keratanase). Biochem J. 1980 Oct 1;191(1):193–207. doi: 10.1042/bj1910193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Poole A. R. Proteoglycans in health and disease: structures and functions. Biochem J. 1986 May 15;236(1):1–14. doi: 10.1042/bj2360001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scott J. E. Proteoglycan-fibrillar collagen interactions. Biochem J. 1988 Jun 1;252(2):313–323. doi: 10.1042/bj2520313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vasios G., Nishimura I., Konomi H., van der Rest M., Ninomiya Y., Olsen B. R. Cartilage type IX collagen-proteoglycan contains a large amino-terminal globular domain encoded by multiple exons. J Biol Chem. 1988 Feb 15;263(5):2324–2329. [PubMed] [Google Scholar]
  16. Vaughan L., Mendler M., Huber S., Bruckner P., Winterhalter K. H., Irwin M. I., Mayne R. D-periodic distribution of collagen type IX along cartilage fibrils. J Cell Biol. 1988 Mar;106(3):991–997. doi: 10.1083/jcb.106.3.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vogel K. G., Paulsson M., Heinegård D. Specific inhibition of type I and type II collagen fibrillogenesis by the small proteoglycan of tendon. Biochem J. 1984 Nov 1;223(3):587–597. doi: 10.1042/bj2230587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wotton S. F., Duance V. C., Fryer P. R. Type IX collagen: a possible function in articular cartilage. FEBS Lett. 1988 Jul 4;234(1):79–82. doi: 10.1016/0014-5793(88)81307-3. [DOI] [PubMed] [Google Scholar]
  19. Wright D. W., Mayne R. Vitreous humor of chicken contains two fibrillar systems: an analysis of their structure. J Ultrastruct Mol Struct Res. 1988 Sep;100(3):224–234. doi: 10.1016/0889-1605(88)90039-0. [DOI] [PubMed] [Google Scholar]
  20. Yada T., Suzuki S., Kobayashi K., Kobayashi M., Hoshino T., Horie K., Kimata K. Occurrence in chick embryo vitreous humor of a type IX collagen proteoglycan with an extraordinarily large chondroitin sulfate chain and short alpha 1 polypeptide. J Biol Chem. 1990 Apr 25;265(12):6992–6999. [PubMed] [Google Scholar]
  21. van der Rest M., Mayne R. Type IX collagen proteoglycan from cartilage is covalently cross-linked to type II collagen. J Biol Chem. 1988 Feb 5;263(4):1615–1618. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES