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. 1991 Nov 1;279(Pt 3):741–745. doi: 10.1042/bj2790741

The role of selenium in the secretion of very-low-density lipoprotein in the isolated perfused rat liver.

R L Scott 1, A Kheshti 1, M Heimberg 1, H G Wilcox 1, W L Stone 1
PMCID: PMC1151508  PMID: 1953666

Abstract

A recirculating liver perfusion system was used to study the effects of dietary selenium (Se) on the hepatic secretion of very-low-density lipoprotein (VLDL). The perfusate from livers of rats fed on a Se-deficient diet incorporated about 50% more [1-14C]oleic acid into triacylglycerol (TG) and cholesteryl esters (ChoEs) than did the perfusate from livers of rats fed on a Se-supplemented diet. Similarly, livers from rats fed the Se-deficient diet secreted more VLDL and incorporated about 60% more [1-14C]oleic acid into VLDL TG and ChoEs than did livers from rats fed the Se-supplemented diet. The liver perfusate from rats in the Se-deficient group also showed significantly decreased fatty acid oxidation. We conclude that Se is a potent modulator of lipoprotein metabolism. A primary action of Se deficiency appears to be a decrease in fatty acid oxidation and a stimulation of fatty acid esterification, leading to increased VLDL TG and ChoEs formation and secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Christiansen R. Z. Regulation of palmitate metabolism by carnitine and glucagon in hepatocytes isolated from fasted and carbohydrate refed rats. Biochim Biophys Acta. 1977 Aug 24;488(2):249–262. doi: 10.1016/0005-2760(77)90182-5. [DOI] [PubMed] [Google Scholar]
  2. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  3. Heimberg M., Wilcox H. G. The effect of palmitic and oleic acids on the properties and composition of the very low density lipoprotein secreted by the liver. J Biol Chem. 1972 Feb 10;247(3):875–880. [PubMed] [Google Scholar]
  4. Katz M. L., Stone W. L., Dratz E. A. Fluorescent pigment accumulation in retinal pigment epithelium of antioxidant-deficient rats. Invest Ophthalmol Vis Sci. 1978 Nov;17(11):1049–1058. [PubMed] [Google Scholar]
  5. Keyes W. G., Wilcox H. G., Heimberg M. Formation of the very low density lipoprotein and metabolism of [1-14C]-oleate by perfused livers from rats treated with triiodothyronine or propylthiouracil. Metabolism. 1981 Feb;30(2):135–146. doi: 10.1016/0026-0495(81)90162-1. [DOI] [PubMed] [Google Scholar]
  6. Kohout M., Kohoutova B., Heimberg M. The regulation of hepatic triglyceride metabolism by free fatty acids. J Biol Chem. 1971 Aug 25;246(16):5067–5074. [PubMed] [Google Scholar]
  7. Little C., O'Brien P. J. An intracellular GSH-peroxidase with a lipid peroxide substrate. Biochem Biophys Res Commun. 1968 Apr 19;31(2):145–150. doi: 10.1016/0006-291x(68)90721-3. [DOI] [PubMed] [Google Scholar]
  8. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  9. Moore J. A., Noiva R., Wells I. C. Selenium concentrations in plasma of patients with arteriographically defined coronary atherosclerosis. Clin Chem. 1984 Jul;30(7):1171–1173. [PubMed] [Google Scholar]
  10. Murthy M. S., Pande S. V. Malonyl-CoA binding site and the overt carnitine palmitoyltransferase activity reside on the opposite sides of the outer mitochondrial membrane. Proc Natl Acad Sci U S A. 1987 Jan;84(2):378–382. doi: 10.1073/pnas.84.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PEARSON D. J., TUBBS P. K. TISSUE LEVELS OF ACID-INSOLUBLE CARNITINE IN RAT HEART. Biochim Biophys Acta. 1964 Dec 2;84:772–773. doi: 10.1016/0926-6542(64)90042-3. [DOI] [PubMed] [Google Scholar]
  12. Paglia D. E., Valentine W. N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967 Jul;70(1):158–169. [PubMed] [Google Scholar]
  13. Rotruck J. T., Pope A. L., Ganther H. E., Swanson A. B., Hafeman D. G., Hoekstra W. G. Selenium: biochemical role as a component of glutathione peroxidase. Science. 1973 Feb 9;179(4073):588–590. doi: 10.1126/science.179.4073.588. [DOI] [PubMed] [Google Scholar]
  14. Rouser G., Siakotos A. N., Fleischer S. Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots. Lipids. 1966 Jan;1(1):85–86. doi: 10.1007/BF02668129. [DOI] [PubMed] [Google Scholar]
  15. Rudel L. L., Morris M. D. Determination of cholesterol using o-phthalaldehyde. J Lipid Res. 1973 May;14(3):364–366. [PubMed] [Google Scholar]
  16. Salam W. H., Wilcox H. G., Heimberg M. Effects of oleic acid on the biosynthesis of lipoprotein apoproteins and distribution into the very-low-density lipoprotein by the isolated perfused rat liver. Biochem J. 1988 May 1;251(3):809–816. doi: 10.1042/bj2510809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Salonen J. T., Salonen R., Seppänen K., Kantola M., Parviainen M., Alfthan G., Mäenpä P. H., Taskinen E., Rauramaa R. Relationship of serum selenium and antioxidants to plasma lipoproteins, platelet aggregability and prevalent ischaemic heart disease in Eastern Finnish men. Atherosclerosis. 1988 Mar;70(1-2):155–160. doi: 10.1016/0021-9150(88)90109-8. [DOI] [PubMed] [Google Scholar]
  18. Stajner A., Sůva J. The determination of nonesterified fatty acids in blood serum using a stable cupric reagent. J Clin Chem Clin Biochem. 1977 Sep;15(9):513–514. doi: 10.1515/cclm.1977.15.1-12.513. [DOI] [PubMed] [Google Scholar]
  19. Stakkestad J. A., Bremer J. The metabolism of fatty acids in hepatocytes isolated from triiodothyronine-treated rats. Biochim Biophys Acta. 1982 Apr 15;711(1):90–100. doi: 10.1016/0005-2760(82)90013-3. [DOI] [PubMed] [Google Scholar]
  20. Steinberg D., Parthasarathy S., Carew T. E., Khoo J. C., Witztum J. L. Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. N Engl J Med. 1989 Apr 6;320(14):915–924. doi: 10.1056/NEJM198904063201407. [DOI] [PubMed] [Google Scholar]
  21. Stone W. L., Stewart M. E., Nicholas C., Pavuluri S. Effects of dietary selenium and vitamin E on plasma lipoprotein cholesterol levels in male rats. Ann Nutr Metab. 1986;30(2):94–103. doi: 10.1159/000177181. [DOI] [PubMed] [Google Scholar]
  22. Stringer M. D., Görög P. G., Freeman A., Kakkar V. V. Lipid peroxides and atherosclerosis. BMJ. 1989 Feb 4;298(6669):281–284. doi: 10.1136/bmj.298.6669.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wilcox H. G., Heimberg M. Secretion and uptake of nascent hepatic very low density lipoprotein by perfused livers from fed and fasted rats. J Lipid Res. 1987 Apr;28(4):351–360. [PubMed] [Google Scholar]
  24. Zakowski J. J., Tappel A. L. Purification and properties of rat liver mitochondrial glutathione peroxidase. Biochim Biophys Acta. 1978 Sep 11;526(1):65–76. doi: 10.1016/0005-2744(78)90290-5. [DOI] [PubMed] [Google Scholar]

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