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. 1991 Oct 1;279(Pt 1):315–318. doi: 10.1042/bj2790315

Expression of epidermal growth factor and its mRNA in pig kidney, pancreas and other tissues.

T J Vaughan 1, J C Pascall 1, P S James 1, K D Brown 1
PMCID: PMC1151583  PMID: 1930151

Abstract

The levels of epidermal growth factor (EGF) mRNA in a wide range of pig tissues were measured by a RNAase-protection assay. The highest levels were found in kidney and pancreas, with lower levels in submaxillary gland, prostate gland and seminal vesicles. Immunocytochemical staining using an antiserum raised against recombinant pig EGF localized expression of the factor to the distal convoluted tubules of the kidney and to the epithelial cells lining the pancreatic and salivary ducts. The observed expression of EGF mRNA and EGF in pig tissues is consistent with a possible role for EGF in the maintenance and repair of the epithelial lining of various ductal systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barton C. M., Hall P. A., Hughes C. M., Gullick W. J., Lemoine N. R. Transforming growth factor alpha and epidermal growth factor in human pancreatic cancer. J Pathol. 1991 Feb;163(2):111–116. doi: 10.1002/path.1711630206. [DOI] [PubMed] [Google Scholar]
  2. Bell G. I., Fong N. M., Stempien M. M., Wormsted M. A., Caput D., Ku L. L., Urdea M. S., Rall L. B., Sanchez-Pescador R. Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization. Nucleic Acids Res. 1986 Nov 11;14(21):8427–8446. doi: 10.1093/nar/14.21.8427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Capecchi M. R. Altering the genome by homologous recombination. Science. 1989 Jun 16;244(4910):1288–1292. doi: 10.1126/science.2660260. [DOI] [PubMed] [Google Scholar]
  4. Carpenter G., Cohen S. Epidermal growth factor. J Biol Chem. 1990 May 15;265(14):7709–7712. [PubMed] [Google Scholar]
  5. Carpenter G., Cohen S. Epidermal growth factor. Annu Rev Biochem. 1979;48:193–216. doi: 10.1146/annurev.bi.48.070179.001205. [DOI] [PubMed] [Google Scholar]
  6. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  7. D'Cruz O. J., Haas G. G., Jr Immunoreactive human epidermal growth factor in human seminal plasma. J Clin Endocrinol Metab. 1989 Jun;68(6):1136–1140. doi: 10.1210/jcem-68-6-1136. [DOI] [PubMed] [Google Scholar]
  8. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  9. Fisher D. A., Lakshmanan J. Metabolism and effects of epidermal growth factor and related growth factors in mammals. Endocr Rev. 1990 Aug;11(3):418–442. doi: 10.1210/edrv-11-3-418. [DOI] [PubMed] [Google Scholar]
  10. Gregory H., Willshire I. R., Kavanagh J. P., Blacklock N. J., Chowdury S., Richards R. C. Urogastrone-epidermal growth factor concentrations in prostatic fluid of normal individuals and patients with benign prostatic hypertrophy. Clin Sci (Lond) 1986 Apr;70(4):359–363. doi: 10.1042/cs0700359. [DOI] [PubMed] [Google Scholar]
  11. Gresik E. W., Barka T. Immunocytochemical localization of epidermal growth factor during the postnatal development of the submandibular gland of the mouse. Am J Anat. 1978 Jan;151(1):1–9. doi: 10.1002/aja.1001510102. [DOI] [PubMed] [Google Scholar]
  12. Hirata Y., Uchihashi M., Nakajima M., Fujita T., Matsukura S. Immunoreactive human epidermal growth factor in human pancreatic juice. J Clin Endocrinol Metab. 1982 Jun;54(6):1242–1245. doi: 10.1210/jcem-54-6-1242. [DOI] [PubMed] [Google Scholar]
  13. Massagué J. Transforming growth factor-alpha. A model for membrane-anchored growth factors. J Biol Chem. 1990 Dec 15;265(35):21393–21396. [PubMed] [Google Scholar]
  14. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pascall J. C., Jones D. S., Doel S. M., Clements J. M., Hunter M., Fallon T., Edwards M., Brown K. D. Cloning and characterization of a gene encoding pig epidermal growth factor. J Mol Endocrinol. 1991 Feb;6(1):63–70. doi: 10.1677/jme.0.0060063. [DOI] [PubMed] [Google Scholar]
  16. Rall L. B., Scott J., Bell G. I., Crawford R. J., Penschow J. D., Niall H. D., Coghlan J. P. Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues. Nature. 1985 Jan 17;313(5999):228–231. doi: 10.1038/313228a0. [DOI] [PubMed] [Google Scholar]
  17. Salido E. C., Fisher D. A., Barajas L. Immunoelectron microscopy of epidermal growth factor in mouse kidney. J Ultrastruct Mol Struct Res. 1986 Jul-Sep;96(1-3):105–113. doi: 10.1016/0889-1605(86)90011-x. [DOI] [PubMed] [Google Scholar]
  18. Samani N. J., Swales J. D., Brammar W. J. Expression of the renin gene in extra-renal tissues of the rat. Biochem J. 1988 Aug 1;253(3):907–910. doi: 10.1042/bj2530907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Skov Olsen P. Role of epidermal growth factor in gastroduodenal mucosal protection. J Clin Gastroenterol. 1988;10 (Suppl 1):S146–S151. doi: 10.1097/00004836-198812001-00022. [DOI] [PubMed] [Google Scholar]
  20. Ullrich A., Gray A., Berman C., Dull T. J. Human beta-nerve growth factor gene sequence highly homologous to that of mouse. Nature. 1983 Jun 30;303(5920):821–825. doi: 10.1038/303821a0. [DOI] [PubMed] [Google Scholar]
  21. Zabel B. U., Eddy R. L., Lalley P. A., Scott J., Bell G. I., Shows T. B. Chromosomal locations of the human and mouse genes for precursors of epidermal growth factor and the beta subunit of nerve growth factor. Proc Natl Acad Sci U S A. 1985 Jan;82(2):469–473. doi: 10.1073/pnas.82.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]

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