Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1991 Oct 1;279(Pt 1):323–326. doi: 10.1042/bj2790323

The identification and purification of the heterotrimeric GTP-binding protein from squid (Loligo forbesi) photoreceptors.

J D Pottinger 1, N J Ryba 1, J N Keen 1, J B Findlay 1
PMCID: PMC1151585  PMID: 1930153

Abstract

The principal GTP-binding protein (G-protein) from squid (Loligo forbesi) photoreceptor membranes has been identified by amino acid sequencing. The heterotrimeric protein was purified by detergent solubilization and ion-exchange chromatography. The amino acid sequence of the G-protein alpha-subunit (G-alpha) indicates that this subunit is closely related to the recently characterized Gq subgroup, whereas the G-gamma subunit varies widely in sequence from other homologues.

Full text

PDF
323

Images in this article

324Fig. 1.
on p.324
324Fig. 2.
on p.324

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baehr W., Morita E. A., Swanson R. J., Applebury M. L. Characterization of bovine rod outer segment G-protein. J Biol Chem. 1982 Jun 10;257(11):6452–6460. [PubMed] [Google Scholar]
  2. Baer K. M., Saibil H. R. Light- and GTP-activated hydrolysis of phosphatidylinositol bisphosphate in squid photoreceptor membranes. J Biol Chem. 1988 Jan 5;263(1):17–20. [PubMed] [Google Scholar]
  3. Bloomquist B. T., Shortridge R. D., Schneuwly S., Perdew M., Montell C., Steller H., Rubin G., Pak W. L. Isolation of a putative phospholipase C gene of Drosophila, norpA, and its role in phototransduction. Cell. 1988 Aug 26;54(5):723–733. doi: 10.1016/s0092-8674(88)80017-5. [DOI] [PubMed] [Google Scholar]
  4. Brown J. E., Rubin L. J., Ghalayini A. J., Tarver A. P., Irvine R. F., Berridge M. J., Anderson R. E. myo-Inositol polyphosphate may be a messenger for visual excitation in Limulus photoreceptors. Nature. 1984 Sep 13;311(5982):160–163. doi: 10.1038/311160a0. [DOI] [PubMed] [Google Scholar]
  5. Cerione R. A., Regan J. W., Nakata H., Codina J., Benovic J. L., Gierschik P., Somers R. L., Spiegel A. M., Birnbaumer L., Lefkowitz R. J. Functional reconstitution of the alpha 2-adrenergic receptor with guanine nucleotide regulatory proteins in phospholipid vesicles. J Biol Chem. 1986 Mar 15;261(8):3901–3909. [PubMed] [Google Scholar]
  6. Devary O., Heichal O., Blumenfeld A., Cassel D., Suss E., Barash S., Rubinstein C. T., Minke B., Selinger Z. Coupling of photoexcited rhodopsin to inositol phospholipid hydrolysis in fly photoreceptors. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6939–6943. doi: 10.1073/pnas.84.19.6939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fein A. Blockade of visual excitation and adaptation in Limulus photoreceptor by GDP-beta-S. Science. 1986 Jun 20;232(4757):1543–1545. doi: 10.1126/science.3487116. [DOI] [PubMed] [Google Scholar]
  8. Fein A., Payne R., Corson D. W., Berridge M. J., Irvine R. F. Photoreceptor excitation and adaptation by inositol 1,4,5-trisphosphate. Nature. 1984 Sep 13;311(5982):157–160. doi: 10.1038/311157a0. [DOI] [PubMed] [Google Scholar]
  9. Fung B. K. Characterization of transducin from bovine retinal rod outer segments. I. Separation and reconstitution of the subunits. J Biol Chem. 1983 Sep 10;258(17):10495–10502. [PubMed] [Google Scholar]
  10. Fyles J. M., Baverstock J., Baer K., Saibil H. R. Effects of calcium on light-activated GTP-binding proteins in squid photoreceptor membranes. Comp Biochem Physiol B. 1991;98(2-3):215–221. doi: 10.1016/0305-0491(91)90169-e. [DOI] [PubMed] [Google Scholar]
  11. Gautam N., Northup J., Tamir H., Simon M. I. G protein diversity is increased by associations with a variety of gamma subunits. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7973–7977. doi: 10.1073/pnas.87.20.7973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gilman A. G. G proteins: transducers of receptor-generated signals. Annu Rev Biochem. 1987;56:615–649. doi: 10.1146/annurev.bi.56.070187.003151. [DOI] [PubMed] [Google Scholar]
  13. Hall M. D., Hoon M. A., Ryba N. J., Pottinger J. D., Keen J. N., Saibil H. R., Findlay J. B. Molecular cloning and primary structure of squid (Loligo forbesi) rhodopsin, a phospholipase C-directed G-protein-linked receptor. Biochem J. 1991 Feb 15;274(Pt 1):35–40. doi: 10.1042/bj2740035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Halliday K. R. Regional homology in GTP-binding proto-oncogene products and elongation factors. J Cyclic Nucleotide Protein Phosphor Res. 1983;9(6):435–448. [PubMed] [Google Scholar]
  15. Kühn H. Light- and GTP-regulated interaction of GTPase and other proteins with bovine photoreceptor membranes. Nature. 1980 Feb 7;283(5747):587–589. doi: 10.1038/283587a0. [DOI] [PubMed] [Google Scholar]
  16. Lee Y. J., Dobbs M. B., Verardi M. L., Hyde D. R. dgq: a drosophila gene encoding a visual system-specific G alpha molecule. Neuron. 1990 Dec;5(6):889–898. doi: 10.1016/0896-6273(90)90349-k. [DOI] [PubMed] [Google Scholar]
  17. Levine M. A., Smallwood P. M., Moen P. T., Jr, Helman L. J., Ahn T. G. Molecular cloning of beta 3 subunit, a third form of the G protein beta-subunit polypeptide. Proc Natl Acad Sci U S A. 1990 Mar;87(6):2329–2333. doi: 10.1073/pnas.87.6.2329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lochrie M. A., Simon M. I. G protein multiplicity in eukaryotic signal transduction systems. Biochemistry. 1988 Jul 12;27(14):4957–4965. doi: 10.1021/bi00414a001. [DOI] [PubMed] [Google Scholar]
  19. O'Tousa J. E., Baehr W., Martin R. L., Hirsh J., Pak W. L., Applebury M. L. The Drosophila ninaE gene encodes an opsin. Cell. 1985 Apr;40(4):839–850. doi: 10.1016/0092-8674(85)90343-5. [DOI] [PubMed] [Google Scholar]
  20. Ovchinnikov YuA, Abdulaev N. G., Zolotarev A. S., Artamonov I. D., Bespalov I. A., Dergachev A. E., Tsuda M. Octopus rhodopsin. Amino acid sequence deduced from cDNA. FEBS Lett. 1988 May 9;232(1):69–72. doi: 10.1016/0014-5793(88)80388-0. [DOI] [PubMed] [Google Scholar]
  21. Pang I. H., Sternweis P. C. Purification of unique alpha subunits of GTP-binding regulatory proteins (G proteins) by affinity chromatography with immobilized beta gamma subunits. J Biol Chem. 1990 Oct 25;265(30):18707–18712. [PubMed] [Google Scholar]
  22. Robinson P. R., Wood S. F., Szuts E. Z., Fein A., Hamm H. E., Lisman J. E. Light-dependent GTP-binding proteins in squid photoreceptors. Biochem J. 1990 Nov 15;272(1):79–85. doi: 10.1042/bj2720079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ryba N. J., Pottinger J. D., Keen J. N., Findlay J. B. Sequence of the beta-subunit of the phosphatidylinositol-specific phospholipase C-directed GTP-binding protein from squid (Loligo forbesi) photoreceptors. Biochem J. 1991 Jan 1;273(Pt 1):225–228. doi: 10.1042/bj2730225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schägger H., von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987 Nov 1;166(2):368–379. doi: 10.1016/0003-2697(87)90587-2. [DOI] [PubMed] [Google Scholar]
  25. Smrcka A. V., Hepler J. R., Brown K. O., Sternweis P. C. Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq. Science. 1991 Feb 15;251(4995):804–807. doi: 10.1126/science.1846707. [DOI] [PubMed] [Google Scholar]
  26. Staden R. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Res. 1982 May 11;10(9):2951–2961. doi: 10.1093/nar/10.9.2951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Strathmann M., Simon M. I. G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates. Proc Natl Acad Sci U S A. 1990 Dec;87(23):9113–9117. doi: 10.1073/pnas.87.23.9113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Szuts E. Z., Wood S. F., Reid M. S., Fein A. Light stimulates the rapid formation of inositol trisphosphate in squid retinas. Biochem J. 1986 Dec 15;240(3):929–932. doi: 10.1042/bj2400929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Taylor S. J., Smith J. A., Exton J. H. Purification from bovine liver membranes of a guanine nucleotide-dependent activator of phosphoinositide-specific phospholipase C. Immunologic identification as a novel G-protein alpha subunit. J Biol Chem. 1990 Oct 5;265(28):17150–17156. [PubMed] [Google Scholar]
  30. Uhl R., Desel H., Wagner R. Separation and characterisation of light scattering transients from rod outer segments of vertebrate photoreceptors: design and performance of a Multi Angle Flash Photolysis Apparatus (MAFPA). J Biochem Biophys Methods. 1985 May;11(1):31–43. doi: 10.1016/0165-022x(85)90039-9. [DOI] [PubMed] [Google Scholar]
  31. Vandenberg C. A., Montal M. Light-regulated biochemical events in invertebrate photoreceptors. 1. Light-activated guanosinetriphosphatase, guanine nucleotide binding, and cholera toxin catalyzed labeling of squid photoreceptor membranes. Biochemistry. 1984 May 22;23(11):2339–2347. doi: 10.1021/bi00306a003. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES