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. 2024 Oct 7;14(17):6426–6445. doi: 10.7150/thno.100802

Figure 1.

Figure 1

Image acquisition process for CE-MRI and whole-body cryo-imaging of candidate fluorescent contrast agents. (A) Experimental timeline. (B) For each animal, a T1-weighted MRI volume was acquired immediately after administration of the contrast agent cocktail, termed “Early CE-MRI”. (C) Another T1-weighted volume was acquired immediately before euthanasia at the specific evaluation time (10, 40 or 90 min after contrast agent administration), here termed “T.M. CE-MRI”. The specimen was then prepared for cryo-imaging. (D) Illustration of the hyperspectral cryo-imaging instrument which images the specimen block under sequential illumination of multiple sources during sectioning. (E) Representative image slices (12 of approximately 200 shown) from each channel illustrate how the image volume was acquired. (F) A rendered RGB volume with overlays of agent fluorescence plotted as maximum intensity projections (MIPs). (G) Renderings and selected 2-D images of just the head. The top row shows surfaces of the tumor and normal brain regions.