Abstract
Silver-stained two-dimensional polyacrylamide gels of Physarum polycephalum extracts have revealed no major changes in protein accumulation during the synchronous nuclear division cycle. However, one protein, or apparent Mr 32 000 and isoelectric point 4.9, shows a reproducible ten-fold increase in total amount between early G2 phase and metaphase. This protein represents about 0.005% of total plasmodial protein.
Full text
PDF


Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Bravo R., Celis J. E. A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells. J Cell Biol. 1980 Mar;84(3):795–802. doi: 10.1083/jcb.84.3.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elliott S. G., McLaughlin C. S. Rate of macromolecular synthesis through the cell cycle of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4384–4388. doi: 10.1073/pnas.75.9.4384. [DOI] [PMC free article] [PubMed] [Google Scholar]
- John P. C., Lambe C. A., McGookin R., Orr B., Rollins M. J. Poly(A) RNA populations, polypeptide synthesis and macromolecule accumulation in the cell cycle of the eukaryote Chlorella. J Cell Sci. 1982 Jun;55:51–67. doi: 10.1242/jcs.55.1.51. [DOI] [PubMed] [Google Scholar]
- Laffler T. G., Chang M. T., Dove W. F. Periodic synthesis of microtubular proteins in the cell cycle of Physarum. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5000–5004. doi: 10.1073/pnas.78.8.5000. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lutkenhaus J. F., Moore B. A., Masters M., Donachie W. D. Individual proteins are synthesized continuously throughout the Escherichia coli cell cycle. J Bacteriol. 1979 May;138(2):352–360. doi: 10.1128/jb.138.2.352-360.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lörincz A. T., Miller M. J., Xuong N. H., Geiduschek E. P. Identification of proteins whose synthesis is modulated during the cell cycle of Saccharomyces cerevisiae. Mol Cell Biol. 1982 Dec;2(12):1532–1549. doi: 10.1128/mcb.2.12.1532. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merril C. R., Switzer R. C., Van Keuren M. L. Trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4335–4339. doi: 10.1073/pnas.76.9.4335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Milcarek C., Zahn K. The synthesis of ninety proteins including actin throughout the HeLa cell cycle. J Cell Biol. 1978 Dec;79(3):833–838. doi: 10.1083/jcb.79.3.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
- Turnock G., Chambers J., Birch B. Regulation of protein synthesis in the plasmodial phase of Physarum polycephalum. Eur J Biochem. 1981 Dec;120(3):529–534. doi: 10.1111/j.1432-1033.1981.tb05732.x. [DOI] [PubMed] [Google Scholar]

