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. 1983 May 15;212(2):433–437. doi: 10.1042/bj2120433

Platelet-activating factor stimulates phosphatidylinositol turnover in human platelets.

D E MacIntyre, W K Pollock
PMCID: PMC1152064  PMID: 6411067

Abstract

Platelet-activating factor stimulates phosphatidylinositol turnover in human platelets as indicated by [32P]phosphatidate accumulation in platelets pre-labelled with [32P]Pi, and by [3H]phosphatidate accumulation and [3H]phosphatidylinositol loss in platelets pre-labelled with [3H]arachidonate. These effects of platelet-activating factor are direct and are independent of the production and/or release of endogenous platelet agonists such as ADP, 5-hydroxytryptamine and thromboxane A2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benveniste J., Henson P. M., Cochrane C. G. Leukocyte-dependent histamine release from rabbit platelets. The role of IgE, basophils, and a platelet-activating factor. J Exp Med. 1972 Dec 1;136(6):1356–1377. doi: 10.1084/jem.136.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Billah M. M., Lapetina E. G., Cuatrecasas P. Phospholipase A2 activity specific for phosphatidic acid. A possible mechanism for the production of arachidonic acid in platelets. J Biol Chem. 1981 Jun 10;256(11):5399–5403. [PubMed] [Google Scholar]
  3. Chignard M., Le Couedic J. P., Tence M., Vargaftig B. B., Benveniste J. The role of platelet-activating factor in platelet aggregation. Nature. 1979 Jun 28;279(5716):799–800. doi: 10.1038/279799a0. [DOI] [PubMed] [Google Scholar]
  4. Gerrard J. M., Kindom S. E., Peterson D. A., Peller J., Krantz K. E., White J. G. Lysophosphatidic acids. Influence on platelet aggregation and intracellular calcium flux. Am J Pathol. 1979 Aug;96(2):423–438. [PMC free article] [PubMed] [Google Scholar]
  5. Haslam R. J., Davidson M. M., Desjardins J. V. Inhibition of adenylate cyclase by adenosine analogues in preparations of broken and intact human platelets. Evidence for the unidirectional control of platelet function by cyclic AMP. Biochem J. 1978 Oct 15;176(1):83–95. doi: 10.1042/bj1760083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lapetina E. G., Cuatrecasas P. Stimulation of phosphatidic acid production in platelets precedes the formation of arachidonate and parallels the release of serotonin. Biochim Biophys Acta. 1979 May 25;573(2):394–402. doi: 10.1016/0005-2760(79)90072-9. [DOI] [PubMed] [Google Scholar]
  7. Lee T. C., Malone B., Blank M. L., Snyder F. 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) stimulates calcium influx in rabbit platelets. Biochem Biophys Res Commun. 1981 Oct 30;102(4):1262–1268. doi: 10.1016/s0006-291x(81)80147-7. [DOI] [PubMed] [Google Scholar]
  8. Lloyd J. V., Nishizawa E. E., Haldar J., Mustard J. F. Changes in 32 p-labelling of platelet phospholipids in response to ADP. Br J Haematol. 1972 Nov;23(5):571–585. doi: 10.1111/j.1365-2141.1972.tb07092.x. [DOI] [PubMed] [Google Scholar]
  9. Lloyd J. V., Nishizawa E. E., Mustard J. F. Effect of ADP-induced shape change on incorporation of 32P into platelet phosphatidic acid and mono-, di- and triphosphatidyl inositol. Br J Haematol. 1973 Jul;25(1):77–99. doi: 10.1111/j.1365-2141.1973.tb01718.x. [DOI] [PubMed] [Google Scholar]
  10. Lote K., Lowery C. Triglyceride lipase activity and human platelets. Thromb Haemost. 1979 Apr 23;41(2):407–415. [PubMed] [Google Scholar]
  11. MacIntyre D. E. Modulation of platelet function by prostaglandins: characterization of platelet receptors for stimulatory prostaglandins and the role of arachidonate metabolites in platelet degranulation responses. Haemostasis. 1979;8(3-5):274–293. doi: 10.1159/000214318. [DOI] [PubMed] [Google Scholar]
  12. MacIntyre D. E., Salzman E. W., Gordon J. L. Prostaglandin receptors on human platelets. Structure-activity relationships of stimulatory prostaglandins. Biochem J. 1978 Sep 15;174(3):921–929. doi: 10.1042/bj1740921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mauco G., Chap H., Douste-Blazy L. Characterization and properties of a phosphatidylinositol phosphodiesterase (phospholipase C) from platelet cytosol. FEBS Lett. 1979 Apr 15;100(2):367–370. doi: 10.1016/0014-5793(79)80371-3. [DOI] [PubMed] [Google Scholar]
  14. Michell R. H. Inositol phospholipids and cell surface receptor function. Biochim Biophys Acta. 1975 Mar 25;415(1):81–47. doi: 10.1016/0304-4157(75)90017-9. [DOI] [PubMed] [Google Scholar]
  15. Rittenhouse-Simmons S. Differential activation of platelet phospholipases by thrombin and ionophore A23187. J Biol Chem. 1981 May 10;256(9):4153–4155. [PubMed] [Google Scholar]
  16. Rittenhouse-Simmons S. Production of diglyceride from phosphatidylinositol in activated human platelets. J Clin Invest. 1979 Apr;63(4):580–587. doi: 10.1172/JCI109339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shaw J. O., Klusick S. J., Hanahan D. J. Activation of rabbit platelet phospholipase and thromboxane synthesis by 1-O-hexadecyl/octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (platelet activating factor). Biochim Biophys Acta. 1981 Jan 26;663(1):222–229. doi: 10.1016/0005-2760(81)90208-3. [DOI] [PubMed] [Google Scholar]
  18. Shukla S. D., Hanahan D. J. AGEPC (platelet activating factor) induced stimulation of rabbit platelets: effects on phosphatidylinositol, di- and triphosphoinositides and phosphatidic acid metabolism. Biochem Biophys Res Commun. 1982 Jun 15;106(3):697–703. doi: 10.1016/0006-291x(82)91767-3. [DOI] [PubMed] [Google Scholar]
  19. Tyson C. A., Vande Zande H., Green D. E. Phospholipids as ionophores. J Biol Chem. 1976 Mar 10;251(5):1326–1332. [PubMed] [Google Scholar]
  20. Vargaftig B. B., Chignard M., Benveniste J. Present concepts on the mechanisms of platelet aggregation. Biochem Pharmacol. 1981 Feb 15;30(4):263–271. doi: 10.1016/0006-2952(81)90052-6. [DOI] [PubMed] [Google Scholar]
  21. Yavin E., Zutra A. Separation and analysis of 32P-labeled phospholipids by a simple and rapid thin-layer chromatographic procedure and its application to cultured neuroblastoma cells. Anal Biochem. 1977 Jun;80(2):430–437. doi: 10.1016/0003-2697(77)90665-0. [DOI] [PubMed] [Google Scholar]

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