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. 1983 Aug 1;213(2):297–304. doi: 10.1042/bj2130297

Evidence for the presence and structure of asparagine-linked oligosaccharide units in the core protein of proteodermatan sulphate.

H Shinkai, T Nakamura, E Matsunaga
PMCID: PMC1152128  PMID: 6615437

Abstract

The N-glycosidically linked oligosaccharides were liberated by hydrazinolysis from purified proteodermatan sulphate from newborn-calf skin and reduced with NaB3H4 at the reducing terminal sugar. One asparagine-linked oligosaccharide chain was linked to one core-protein molecule in proteodermatan sulphate. Structural sequences were analysed by using exoglycosidase digestion. These oligosaccharides were composed of di- and tri-antennary oligosaccharide structures of complex type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arakawa M., Ogata S., Muramatsu T., Kobata A. Beta-galactosidases from jack bean meal and almond emulsin. Application for the enzymatic distinction of Galbeta1 leads to 4GlcNAc and Galbeta1 leads to 3GlcNAc linkages. J Biochem. 1974 Apr;75(4):707–714. doi: 10.1093/oxfordjournals.jbchem.a130443. [DOI] [PubMed] [Google Scholar]
  2. Cöster L., Fransson L. A. Isolation and characterization of dermatan sulphate proteoglycans from bovine sclera. Biochem J. 1981 Jan 1;193(1):143–153. doi: 10.1042/bj1930143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. De Luca S., Lohmander L. S., Nilsson B., Hascall V. C., Caplan A. I. Proteoglycans from chick limb bud chondrocyte cultures. Keratan sulfate and oligosaccharides which contain mannose and sialic acid. J Biol Chem. 1980 Jul 10;255(13):6077–6083. [PubMed] [Google Scholar]
  4. Fleischmajer R., Perlish J. S., Bashey R. I. Human dermal glycosaminoglycans and aging. Biochim Biophys Acta. 1972 Sep 15;279(2):265–275. doi: 10.1016/0304-4165(72)90142-0. [DOI] [PubMed] [Google Scholar]
  5. Fujii N., Nagai Y. Isolation and characterization of a proteodermatan sulfate from calf skin. J Biochem. 1981 Nov;90(5):1249–1258. doi: 10.1093/oxfordjournals.jbchem.a133589. [DOI] [PubMed] [Google Scholar]
  6. HUGHES R. C., JEANLOZ R. W. THE EXTRACELLULAR GLYCOSIDASES OF DIPLOCOCCUS PNEUMONIAE. I. PURIFICATION AND PROPERTIES OF A NEURAMINIDASE AND A BETA-GALACTOSIDASE. ACTION ON THE ALPHA-1-ACID GLYCOPROTEIN OF HUMAN PLASMA. Biochemistry. 1964 Oct;3:1535–1543. doi: 10.1021/bi00898a025. [DOI] [PubMed] [Google Scholar]
  7. Kobata A. Use of endo- and exoglycosidases for structural studies of glycoconjugates. Anal Biochem. 1979 Nov 15;100(1):1–14. doi: 10.1016/0003-2697(79)90102-7. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lohmander L. S., De Luca S., Nilsson B., Hascall V. C., Caputo C. B., Kimura J. H., Heinegard D. Oligosaccharides on proteoglycans from the swarm rat chondrosarcoma. J Biol Chem. 1980 Jul 10;255(13):6084–6091. [PubMed] [Google Scholar]
  10. Mizuochi T., Taniguchi T., Fujii-Kadowaki J., Yonemasu K., Sasaki T., Kobata A. Structures of the asparagine-linked sugar chains of subcomponent C1q of the first component of bovine complement. J Biol Chem. 1982 Nov 25;257(22):13300–13305. [PubMed] [Google Scholar]
  11. Mizuochi T., Yonemasu K., Yamashita K., Kobata A. The asparagine-linked sugar chains of subcomponent C1q of the first component of human complement. J Biol Chem. 1978 Oct 25;253(20):7404–7409. [PubMed] [Google Scholar]
  12. Nakamura T., Matsunaga E., Shinkai H. Isolation and some structural analyses of a proteodermatan sulphate from calf skin. Biochem J. 1983 Aug 1;213(2):289–296. doi: 10.1042/bj2130289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nilsson B., De Luca S., Lohmander S., Hascall V. C. Structures of N-linked and O-linked oligosaccharides on proteoglycan monomer isolated from the Swarm rat chondrosarcoma. J Biol Chem. 1982 Sep 25;257(18):10920–10927. [PubMed] [Google Scholar]
  14. Nishigaki M., Yamashita K., Matsuda I., Arashima S., Kobata A. Urinary oligosaccharides of fucosidosis. Evidence of the occurrence of X-antigenic determinant in serum-type sugar chains of glycoproteins. J Biochem. 1978 Oct;84(4):823–834. doi: 10.1093/oxfordjournals.jbchem.a132194. [DOI] [PubMed] [Google Scholar]
  15. Scott J. E., Orford C. R. Dermatan sulphate-rich proteoglycan associates with rat tail-tendon collagen at the d band in the gap region. Biochem J. 1981 Jul 1;197(1):213–216. doi: 10.1042/bj1970213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Takasaki S., Kobata A. Microdetermination of individual neutral and amino sugars and N-acetylneuraminic acid in complex saccharides. J Biochem. 1974 Oct;76(4):783–789. [PubMed] [Google Scholar]
  17. Yoshima H., Takasaki S., Kobata A. The asparagine-linked sugar chains of the glycoproteins in calf thymocyte plasma membrane. Structural studies of acidic oligosaccharides. J Biol Chem. 1980 Nov 25;255(22):10793–10804. [PubMed] [Google Scholar]

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