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. 1983 Aug 1;213(2):313–319. doi: 10.1042/bj2130313

Studies on the structure of a phosphoglycoprotein from the parasitic protozoan Trypanosoma cruzi.

M A Ferguson, A K Allen, D Snary
PMCID: PMC1152130  PMID: 6351838

Abstract

A glycoprotein (GP72) has been isolated from Trypanosoma cruzi and found to contain 41% protein, 49% carbohydrate and 10% phosphate. All phosphate was covalently attached to the carbohydrate which contained the following sugars: ribose, xylose, fucose, galactose, mannose, glucose and glucosamine. The carbohydrate side chains were linked to protein by fucose, xylose and N-acetylglucosamine; 50% of the total N-acetylglucosamine was involved in glycoprotein linkages. Two classes of carbohydrate side chains were detected. One class comprised 15% of the total carbohydrate and contained glucosamine, mannose and galactose; some of these chains were phosphorylated. The other class comprised 85% of the total carbohydrate and contained xylose, ribose, fucose, galactose, mannose, glucosamine and phosphate; these chains were antigenic and reacted with a monoclonal antibody with specificity for the whole glycoprotein.

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Selected References

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  1. Allen A. K., Desai N. N., Neuberger A. Purification of the glycoprotein lectin from the broad bean (Vicia faba) and a comparison of its properties with lectins of similar specificity. Biochem J. 1976 Apr 1;155(1):127–135. doi: 10.1042/bj1550127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allen A. K., Neuberger A. The quantitation of glucosamine and galactosamine in glycoproteins after hydrolysis in p-toluenesulphonic acid. FEBS Lett. 1975 Dec 1;60(1):76–80. doi: 10.1016/0014-5793(75)80422-4. [DOI] [PubMed] [Google Scholar]
  3. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  4. Brener Z. Biology of Trypanosoma cruzi. Annu Rev Microbiol. 1973;27:347–382. doi: 10.1146/annurev.mi.27.100173.002023. [DOI] [PubMed] [Google Scholar]
  5. Brener Z. Recent developments in the field of Chagas' disease. Bull World Health Organ. 1982;60(4):463–473. [PMC free article] [PubMed] [Google Scholar]
  6. Chambers R. E., Clamp J. R. An assessment of methanolysis and other factors used in the analysis of carbohydrate-containing materials. Biochem J. 1971 Dec;125(4):1009–1018. doi: 10.1042/bj1251009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gorin P. A., Previato J. O., Mendonça-Previato L., Travassos L. R. Structure of the D-mannan and D-arabino-D-galactan in Crithidia fasciculata: changes in proportion with age of culture. J Protozool. 1979 Aug;26(3):473–478. doi: 10.1111/j.1550-7408.1979.tb04656.x. [DOI] [PubMed] [Google Scholar]
  8. Green J. R., Northcote D. H. The structure and function of glycoproteins synthesized during slime-polysaccharide production by membranes of the root-cap cells of maize (Zea mays). Biochem J. 1978 Mar 15;170(3):599–608. doi: 10.1042/bj1700599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gustafson G. L., Milner L. A. Occurrence of N-acetylglucosamine-1-phosphate in proteinase I from Dictyostelium discoideum. J Biol Chem. 1980 Aug 10;255(15):7208–7210. [PubMed] [Google Scholar]
  10. Hayaishi O., Ueda K. Poly(ADP-ribose) and ADP-ribosylation of proteins. Annu Rev Biochem. 1977;46:95–116. doi: 10.1146/annurev.bi.46.070177.000523. [DOI] [PubMed] [Google Scholar]
  11. Heaney-Kieras J., Rodén L., Chapman D. J. The covalent linkage of protein to carbohydrate in the extracellular protein-polysaccharide from the red alga Porphyridium cruentum. Biochem J. 1977 Jul 1;165(1):1–9. doi: 10.1042/bj1650001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holder A. A., Cross G. A. Glycopeptides from variant surface glycoproteins of Trypanosoma Brucei. C-terminal location of antigenically cross-reacting carbohydrate moieties. Mol Biochem Parasitol. 1981 Feb;2(3-4):135–150. doi: 10.1016/0166-6851(81)90095-5. [DOI] [PubMed] [Google Scholar]
  13. Klinger M. M., Laine R. A., Steiner S. M. Characterization of novel amino acid fucosides. J Biol Chem. 1981 Aug 10;256(15):7932–7935. [PubMed] [Google Scholar]
  14. Lis H., Sharon N. Soybean agglutinin--a plant glycoprotein. Structure of the carboxydrate unit. J Biol Chem. 1978 May 25;253(10):3468–3476. [PubMed] [Google Scholar]
  15. Mega T., Ikenaka T. Methanolysis products of asparagine-linked N-acetylglucosamine and a new method for determination of N- and O-glycosidic N-acetylglucosamine in glycoproteins that contain asparagine-linked carbohydrates. Anal Biochem. 1982 Jan 1;119(1):17–24. doi: 10.1016/0003-2697(82)90659-5. [DOI] [PubMed] [Google Scholar]
  16. Ogata S., Lloyd K. O. Mild alkaline borohydride treatment of glycoproteins-a method for liberating both N- and O-linked carbohydrate chains. Anal Biochem. 1982 Jan 15;119(2):351–359. doi: 10.1016/0003-2697(82)90597-8. [DOI] [PubMed] [Google Scholar]
  17. Sher A., Snary D. Specific inhibition of the morphogenesis of Trypanosoma cruzi by a monoclonal antibody. Nature. 1982 Dec 16;300(5893):639–640. doi: 10.1038/300639a0. [DOI] [PubMed] [Google Scholar]
  18. Snary D. Cell surface glycoproteins of Trypanosoma cruzi: protective immunity in mice and antibody levels in human chagasic sera. Trans R Soc Trop Med Hyg. 1983;77(1):126–129. doi: 10.1016/0035-9203(83)90037-8. [DOI] [PubMed] [Google Scholar]
  19. Varki A., Kornfeld S. Identification of a rat liver alpha-N-acetylglucosaminyl phosphodiesterase capable of removing "blocking" alpha-N-acetylglucosamine residues from phosphorylated high mannose oligosaccharides of lysosomal enzymes. J Biol Chem. 1980 Sep 25;255(18):8398–8401. [PubMed] [Google Scholar]

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