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. 1983 Aug 1;213(2):427–435. doi: 10.1042/bj2130427

The macromolecular structure of human cervical-mucus glycoproteins. Studies on fragments obtained after reduction of disulphide bridges and after subsequent trypsin digestion.

I Carlstedt, H Lindgren, J K Sheehan
PMCID: PMC1152144  PMID: 6615445

Abstract

Human cervical-mucus glycoproteins (mucins) were extracted with 6 M-guanidinium chloride in the presence of proteinase inhibitors and purified by isopycnic density-gradient centrifugation. The whole mucins (Mr approx. 10 X 10(6] were degraded into 'subunits' (Mr approx. 2 X 10(6] by reduction of disulphide bonds. Trypsin digestion of the 'subunits' produced glycopeptides with Mr approx. 380000, which appear to be rod-like with a length of approx. 105 nm. The relationship between the radius of gyration and the Mr value obtained by light-scattering for whole mucins, 'subunits' and 'domains' suggest that cervical-mucus glycoproteins are linear flexible macromolecules composed of, on the average, four or five 'domains'/subunit and four subunits/whole mucin macromolecule. The shape-dependent particle scattering function for the whole mucins and the 'subunits' are in accordance with that of a linear flexible chain. No evidence for a branched or a star-like structure was found. A tentative model for cervical mucins is proposed.

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Selected References

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  1. CASASSA E. F., EISENBERG H. THERMODYNAMIC ANALYSIS OF MULTICOMPONENT SOLUTIONS. Adv Protein Chem. 1964;19:287–395. doi: 10.1016/s0065-3233(08)60191-6. [DOI] [PubMed] [Google Scholar]
  2. Carlstedt I., Lindgren H., Sheehan J. K., Ulmsten U., Wingerup L. Isolation and characterization of human cervical-mucus glycoproteins. Biochem J. 1983 Apr 1;211(1):13–22. doi: 10.1042/bj2110013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clamp J. R. Chemical aspects of mucus. General considerations. Br Med Bull. 1978 Jan;34(1):25–27. doi: 10.1093/oxfordjournals.bmb.a071455. [DOI] [PubMed] [Google Scholar]
  4. Creeth J. M., Bhaskar K. R., Horton J. R., Das I., Lopez-Vidriero M. T., Reid L. The separation and characterization of bronchial glycoproteins by density-gradient methods. Biochem J. 1977 Dec 1;167(3):557–569. doi: 10.1042/bj1670557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feldhoff P. A., Bhavanandan V. P., Davidson E. A. Purification, properties, and analysis of human asthmatic bronchial mucin. Biochemistry. 1979 May 29;18(11):2430–2436. doi: 10.1021/bi00578a044. [DOI] [PubMed] [Google Scholar]
  6. Hatcher V. B., Schwarzmann G. O., Jeanloz R. W., McArthur J. W. Purification, properties, and partial structure elucidation of a high-molecular-weight glycoprotein from cervical mucus of the bonnet monkey (Macaca radiata). Biochemistry. 1977 Apr 5;16(7):1518–1524. doi: 10.1021/bi00626a042. [DOI] [PubMed] [Google Scholar]
  7. Houdret N., Le Treut A., Lhermitte M., Lamblin G., Degand P., Roussel P. Comparative action of reducing agents on fibrillar human bronchial mucus under dissociating and non-dissociating conditions. Biochim Biophys Acta. 1981 May 29;668(3):413–419. doi: 10.1016/0005-2795(81)90175-6. [DOI] [PubMed] [Google Scholar]
  8. Jourdian G. W., Dean L., Roseman S. The sialic acids. XI. A periodate-resorcinol method for the quantitative estimation of free sialic acids and their glycosides. J Biol Chem. 1971 Jan 25;246(2):430–435. [PubMed] [Google Scholar]
  9. Lamblin G., Lhermitte M., Degand P., Roussel P., Slayter H. S. Chemical and physical properties of human bronchial mucus glycoproteins. Biochimie. 1979;61(1):23–43. doi: 10.1016/s0300-9084(79)80310-7. [DOI] [PubMed] [Google Scholar]
  10. Lindahl U. Attempted isolation of a heparin proteoglycan from bovine liver capsule. Biochem J. 1970 Jan;116(1):27–34. doi: 10.1042/bj1160027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lohmander L. S., De Luca S., Nilsson B., Hascall V. C., Caputo C. B., Kimura J. H., Heinegard D. Oligosaccharides on proteoglycans from the swarm rat chondrosarcoma. J Biol Chem. 1980 Jul 10;255(13):6084–6091. [PubMed] [Google Scholar]
  12. Wolf D. P., Sokoloski J., Khan M. A., Litt M. Human cervical mucus. III. Isolation and characterization of rheologically active mucin. Fertil Steril. 1977 Jan;28(1):53–58. doi: 10.1016/s0015-0282(16)42317-4. [DOI] [PubMed] [Google Scholar]
  13. Woodward H., Horsey B., Bhavanandan V. P., Davidson E. A. Isolation, purification, and properties of respiratory mucus glycoproteins. Biochemistry. 1982 Feb 16;21(4):694–701. doi: 10.1021/bi00533a017. [DOI] [PubMed] [Google Scholar]
  14. YPHANTIS D. A. EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS. Biochemistry. 1964 Mar;3:297–317. doi: 10.1021/bi00891a003. [DOI] [PubMed] [Google Scholar]
  15. Yurewicz E. C., Moghissi K. S. Purification of human midcycle cervical mucin and characterization of its oligosaccharides with respect to size, composition, and microheterogeneity. J Biol Chem. 1981 Nov 25;256(22):11895–11904. [PubMed] [Google Scholar]

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