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. 1983 Aug 1;213(2):437–444. doi: 10.1042/bj2130437

Purification of the major endoglucanase from Aspergillus fumigatus Fresenius.

J B Parry, J C Stewart, J Heptinstall
PMCID: PMC1152145  PMID: 6351841

Abstract

Aspergillus fumigatus (Fresenius), IMI 246651, A.T.C.C. 46324, produces two beta-glucosidase enzymes, cotton-solubilizing activity, xylanase and endoglucanase enzymes which can be separated by gel-filtration chromatography. The major endoglucanase does not bind to concanavalin A-Sepharose and does not stain with periodic acid/Schiff reagent. It is homogeneous on polyacrylamide isoelectric focusing (pI = 7.1) and has a mol.wt. of 12500 by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The endoglucanase produces glucose and a mixture of oligosaccharides from cellulose; the purified enzyme has a small dextranase activity. It is stable at 50 degrees C and pH 6.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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