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. 1983 Sep 1;213(3):625–633. doi: 10.1042/bj2130625

Rat liver glutathione S-transferases. A study of the structure of the basic YbYb-containing enzymes.

J D Hayes
PMCID: PMC1152177  PMID: 6615452

Abstract

A purification scheme was devised that resulted in the resolution of a number of basic glutathione S-transferases from rat liver, three of which contained two subunits of molecular mass 23500 Da (i.e. Yb monomers). These were identified as transferases D, C and A by their elution positions from CM-cellulose and their specific activities towards a variety of substrates. Hybridization, immunotitration and peptide 'mapping' experiments demonstrated that transferases D, C and A comprise Yb2Yb2, Yb1Yb2 and Yb1Yb1 subunits.

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Selected References

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