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. 1983 Jul 15;214(1):189–194. doi: 10.1042/bj2140189

Endocytosis of liposomes bound to cell surface proteins measured by flow cytofluorometry.

A Truneh, Z Mishal, J Barbet, P Machy, L D Leserman
PMCID: PMC1152225  PMID: 6615464

Abstract

A new technique for the quantification of cellular receptor-mediated endocytosis has been developed based on the analysis by flow cytometry of ligand-bearing liposomes containing the fluorochrome carboxyfluorescein. Carboxyfluorescein encapsulated at high concentrations in protein A-bearing liposomes is self-quenched. Binding and internalization of such liposomes by cells via antibodies directed towards membrane surface determinants results in the release of the liposome-encapsulated carboxyfluorescein into the cytoplasm causing an increase in cell-associated fluorescence. This increase can be quantified on a flow cytofluorometer.

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Selected References

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