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. 1983 Sep 15;214(3):851–854. doi: 10.1042/bj2140851

The lipid fluidity of rat liver membrane subfractions

Anthony D Whetton *,†, Miles D Houslay ‡, Nicholas J F Dodd *, W Howard Evans §
PMCID: PMC1152323  PMID: 16743791

Abstract

1. The lipid fluidity of three major rat liver plasma-membrane subfractions, as well as Golgi apparatus and endocytic fractions, was assessed with a fatty acid spin probe by using e.s.r. techniques. 2. The sinusoidal (blood-facing) plasma-membrane subfraction was the most fluid of the three plasma-membrane regions. Fractions originating from the bile-canalicular and contiguous (lateral) regions were most rigid. Endocytic fractions isolated (endosomes and diacytosomes) were of a similar fluidity to fractions originating from the sinusoidal plasma-membrane region. By far the most fluid fractions examined were derived from the Golgi-apparatus complex. 3. The three plasma-membrane subfractions each showed a different response to the bilayer-fluidizing effect of benzyl alcohol. 4. Arrhenius-type plots of the order parameter S and outer hyperfine splitting, 2T∥, identified lipid-phase separations in the plasma-membrane subfractions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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