Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1983 Sep 15;214(3):1019–1022. doi: 10.1042/bj2141019

Fructose 2,6-bisphosphate in livers of genetically obese rats.

L Hue, G van de Werve, B Jeanrenaud
PMCID: PMC1152349  PMID: 6226282

Abstract

Livers of genetically obese (fa/fa) rats, starved for 24 h, contained more fructose 2,6-bisphosphate, glucose 6-phosphate, fructose 6-phosphate and glycogen, and more pyruvate kinase and phosphofructokinase 2 activities, than livers of control lean rats. These changes may be explained in terms of cyclic AMP concentration, which was decreased in livers of obese starved rats.

Full text

PDF
1019

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Claus T. H., El-Maghrabi M. R., Pilkis S. J. Modulation of the phosphorylation state of rat liver pyruvate kinase by allosteric effectors and insulin. J Biol Chem. 1979 Aug 25;254(16):7855–7864. [PubMed] [Google Scholar]
  2. Claus T. H., Pilkis S. J. Fructose 2,6-bisphosphate levels are elevated in livers of genetically obese mice. Biochem Biophys Res Commun. 1982 Dec 15;109(3):664–668. doi: 10.1016/0006-291x(82)91991-x. [DOI] [PubMed] [Google Scholar]
  3. Feliú J. E., Hue L., Hers H. G. Hormonal control of pyruvate kinase activity and of gluconeogenesis in isolated hepatocytes. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2762–2766. doi: 10.1073/pnas.73.8.2762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Felíu J. E., Hue L., Hers H. G. Regulation in vitro and in vivo of adenosine 3':5'-monophosphate-dependent inactivation of rat-liver pyruvate kinase type L. Eur J Biochem. 1977 Dec;81(3):609–617. doi: 10.1111/j.1432-1033.1977.tb11988.x. [DOI] [PubMed] [Google Scholar]
  5. Furuya E., Yokoyama M., Uyeda K. Regulation of fructose-6-phosphate 2-kinase by phosphorylation and dephosphorylation: possible mechanism for coordinated control of glycolysis and glycogenolysis. Proc Natl Acad Sci U S A. 1982 Jan;79(2):325–329. doi: 10.1073/pnas.79.2.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herbert V., Lau K. S., Gottlieb C. W., Bleicher S. J. Coated charcoal immunoassay of insulin. J Clin Endocrinol Metab. 1965 Oct;25(10):1375–1384. doi: 10.1210/jcem-25-10-1375. [DOI] [PubMed] [Google Scholar]
  8. Hers H. G., Hue L. Gluconeogenesis and related aspects of glycolysis. Annu Rev Biochem. 1983;52:617–653. doi: 10.1146/annurev.bi.52.070183.003153. [DOI] [PubMed] [Google Scholar]
  9. Hers H. G., Van Schaftingen E. Fructose 2,6-bisphosphate 2 years after its discovery. Biochem J. 1982 Jul 15;206(1):1–12. doi: 10.1042/bj2060001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hue L., Blackmore P. F., Exton J. H. Fructose 2,6-bisphosphate. Hormonal regulation and mechanism of its formation in liver. J Biol Chem. 1981 Sep 10;256(17):8900–8903. [PubMed] [Google Scholar]
  11. Hue L., Bontemps F., Hers H. The effects of glucose and of potassium ions on the interconversion of the two forms of glycogen phosphorylase and of glycogen synthetase in isolated rat liver preparations. Biochem J. 1975 Oct;152(1):105–114. doi: 10.1042/bj1520105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hue L. Role of fructose 2,6-bisphosphate in the stimulation of glycolysis by anoxia in isolated hepatocytes. Biochem J. 1982 Aug 15;206(2):359–365. doi: 10.1042/bj2060359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hue L., van de Werve G. Increased concentration of fructose 2,6-bisphosphate in livers of genetically obese mice. FEBS Lett. 1982 Aug 23;145(2):263–266. doi: 10.1016/0014-5793(82)80179-8. [DOI] [PubMed] [Google Scholar]
  14. Lavine R. L., Voyles N., Perrino P. V., Recant L. The effect of fasting on tissue cyclic cAMP and plasma glucagon in the obese hyperglycemic mouse. Endocrinology. 1975 Sep;97(3):615–620. doi: 10.1210/endo-97-3-615. [DOI] [PubMed] [Google Scholar]
  15. McCune S. A., Durant P. J., Jenkins P. A., Harris R. A. Comparative studies on fatty acid synthesis, glycogen metabolism, and gluconeogenesis by hepatocytes isolated from lean and obese Zucker rats. Metabolism. 1981 Dec;30(12):1170–1178. doi: 10.1016/0026-0495(81)90037-8. [DOI] [PubMed] [Google Scholar]
  16. Spydevold S. O., Greenbaum A. L., Baquer N. Z., McLean P. Adaptive responses of enzymes of carbohydrate and lipid metabolism to dietary alteration in genetically obese Zucker rats (fa/fa). Eur J Biochem. 1978 Sep 1;89(2):329–339. doi: 10.1111/j.1432-1033.1978.tb12534.x. [DOI] [PubMed] [Google Scholar]
  17. Terrettaz J., Jeanrenaud B. In vivo hepatic and peripheral insulin resistance in genetically obese (fa/fa) rats. Endocrinology. 1983 Apr;112(4):1346–1351. doi: 10.1210/endo-112-4-1346. [DOI] [PubMed] [Google Scholar]
  18. Van Schaftingen E., Davies D. R., Hers H. G. Inactivation of phosphofructokinase 2 by cyclic AMP - dependent protein kinase. Biochem Biophys Res Commun. 1981 Nov 16;103(1):362–368. doi: 10.1016/0006-291x(81)91701-0. [DOI] [PubMed] [Google Scholar]
  19. Van Schaftingen E., Lederer B., Bartrons R., Hers H. G. A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate. Eur J Biochem. 1982 Dec;129(1):191–195. doi: 10.1111/j.1432-1033.1982.tb07039.x. [DOI] [PubMed] [Google Scholar]
  20. Van de Werve G., Van den Berghe G., Hers H. G. A simplified procedure for the assay of adenosine 3':5'-monophosphate by the activation of liver phosphorylase. Eur J Biochem. 1974 Jan 3;41(1):97–102. doi: 10.1111/j.1432-1033.1974.tb03248.x. [DOI] [PubMed] [Google Scholar]
  21. WOLLENBERGER A., RISTAU O., SCHOFFA G. [A simple technic for extremely rapid freezing of large pieces of tissue]. Pflugers Arch Gesamte Physiol Menschen Tiere. 1960;270:399–412. [PubMed] [Google Scholar]
  22. el-Maghrabi M. R., Claus T. H., Pilkis J., Pilkis S. J. Regulation of 6-phosphofructo-2-kinase activity by cyclic AMP-dependent phosphorylation. Proc Natl Acad Sci U S A. 1982 Jan;79(2):315–319. doi: 10.1073/pnas.79.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van Berkel T. J., Kruijt J. K., Koster J. F. Hormone-induced changes in pyruvate kinase. Effects of glucagon and starvation. Eur J Biochem. 1977 Dec;81(3):423–432. doi: 10.1111/j.1432-1033.1977.tb11967.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES