Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1983 Oct 15;216(1):121–128. doi: 10.1042/bj2160121

Adenosine and the control of lipolysis in rat adipocytes during pregnancy and lactation.

R G Vernon, E Finley, E Taylor
PMCID: PMC1152478  PMID: 6316932

Abstract

The rate of noradrenaline-stimulated lipolysis is lower in fat-cells from lactating than from pregnant rats; this difference is eliminated by the addition of adenosine deaminase [Aitchison, Clegg & Vernon (1982) Biochem. J. 202, 243-247]. The activity of 5'-nucleotidase, and hence the capacity of the cells to synthesize adenosine, was the same in fat-cells and also stromal cells of adipose tissue from pregnant, lactating and male rats. The response and sensitivity of fat-cells to the anti-lipolytic effects of adenosine were measured by incubating cells in the presence of noradrenaline, adenosine deaminase (to remove endogenous adenosine) and various concentrations of the adenosine analogue N6-phenylisopropyladenosine (PIA). PIA caused a greater inhibition of the rate of noradrenaline-stimulated lipolysis in adipocytes from lactating than from pregnant rats. The concentration of PIA required to inhibit by 50% the rate of noradrenaline-stimulated lipolysis fell from over 100 nM for fat-cells from pregnant rats to 30 nM for fat-cells from lactating rats. The decreased rate of noradrenaline-stimulated lipolysis during lactation was not due to the smaller mean cell volume of adipocytes during this state.

Full text

PDF
121

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aitchison R. E., Clegg R. A., Vernon R. G. Lipolysis in rat adipocytes during pregnancy and lactation. The response to noradrenaline. Biochem J. 1982 Jan 15;202(1):243–247. doi: 10.1042/bj2020243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arch J. R., Newsholme E. A. Activities and some properties of 5'-nucleotidase, adenosine kinase and adenosine deaminase in tissues from vertebrates and invertebrates in relation to the control of the concentration and the physiological role of adenosine. Biochem J. 1978 Sep 15;174(3):965–977. doi: 10.1042/bj1740965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arch J. R., Newsholme E. A. The control of the metabolism and the hormonal role of adenosine. Essays Biochem. 1978;14:82–123. [PubMed] [Google Scholar]
  4. Björntorp P., Karlsson M., Gustafsson L., Smith U., Sjöström L., Cigolini M., Storck G., Pettersson P. Quantitation of different cells in the epididymal fat pad of the rat. J Lipid Res. 1979 Jan;20(1):97–106. [PubMed] [Google Scholar]
  5. Cleary M. P., Brasel J. A., Greenwood M. R. Developmental changes in thymidine kinase, DNA, and fat cellularity in Zucker rats. Am J Physiol. 1979 May;236(5):E508–E513. doi: 10.1152/ajpendo.1979.236.5.E508. [DOI] [PubMed] [Google Scholar]
  6. Flint D. J., Sinnett-Smith P. A., Clegg R. A., Vernon R. G. Role of insulin receptors in the changing metabolism of adipose tissue during pregnancy and lactation in the rat. Biochem J. 1979 Aug 15;182(2):421–427. doi: 10.1042/bj1820421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fredholm B. B. Local regulation of lipolysis in adipose tissue by fatty acids, prostaglandins and adenosine. Med Biol. 1978 Oct;56(5):249–261. [PubMed] [Google Scholar]
  8. Fredholm B. B., Sollevi A. The release of adenosine and inosine from canine subcutaneous adipose tissue by nerve stimulation and noradrenaline. J Physiol. 1981;313:351–367. doi: 10.1113/jphysiol.1981.sp013670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Green A., Fisher M., Newsholme E. A. Maximum activities of enzymes involved in adenosine metabolism in adipose tissue of rats and mice under conditions of variations in insulin sensitivity. Biochim Biophys Acta. 1981 Aug 5;676(1):125–128. doi: 10.1016/0304-4165(81)90018-0. [DOI] [PubMed] [Google Scholar]
  10. Green A., Newsholme E. A. Distribution of adenosine metabolising enzymes between adipocyte and stromal-vascular cells of adipose tissue. Biochim Biophys Acta. 1981 Aug 5;676(1):122–124. doi: 10.1016/0304-4165(81)90017-9. [DOI] [PubMed] [Google Scholar]
  11. Hanson R. W., Ballard F. J. Citrate, pyruvate, and lactate contaminants of commercial serum albumin. J Lipid Res. 1968 Sep;9(5):667–668. [PubMed] [Google Scholar]
  12. Honnor R. C., Saggerson E. D. Altered lipolytic response to glucagon and adenosine deaminase in adipocytes from starved rats. Biochem J. 1980 Jun 15;188(3):757–761. doi: 10.1042/bj1880757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kazdová L., Fábry P., Vrána A. Effect of small doses of insulin in vivo on the proliferation and cellularity of adipose tissue. Diabetologia. 1974 Feb;10(1):77–83. doi: 10.1007/BF00421417. [DOI] [PubMed] [Google Scholar]
  14. Knopp R. H., Saudek C. D., Arky R. A., O'Sullivan J. B. 2 phases of adipose tissue metabolism in pregnancy: maternal adaptations for fetal growth. Endocrinology. 1973 Apr;92(4):984–988. doi: 10.1210/endo-92-4-984. [DOI] [PubMed] [Google Scholar]
  15. Labarca C., Paigen K. A simple, rapid, and sensitive DNA assay procedure. Anal Biochem. 1980 Mar 1;102(2):344–352. doi: 10.1016/0003-2697(80)90165-7. [DOI] [PubMed] [Google Scholar]
  16. Lorenzo M., Caldés T., Benito M., Medina J. M. Lipogenesis in vivo in maternal and foetal tissues during late gestation in the rat. Biochem J. 1981 Aug 15;198(2):425–428. doi: 10.1042/bj1980425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ohisalo J. J., Stouffer J. E. Adenosine, thyroid status and regulation of lipolysis. Biochem J. 1979 Jan 15;178(1):249–251. doi: 10.1042/bj1780249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Saggerson E. D. Increased antilipolytic effect of the adenosine 'R-site' agonist N6-(phenylisopropyl)adenosine in adipocytes from adrenalectomized rats. FEBS Lett. 1980 Jun 16;115(1):127–128. doi: 10.1016/0014-5793(80)80741-1. [DOI] [PubMed] [Google Scholar]
  19. Schwabe U., Ebert R., Erbler H. C. Adenosine release from isolated fat cells and its significance for the effects of hormones on cyclic 3',5'-AMP levels and lipolysis. Naunyn Schmiedebergs Arch Pharmacol. 1973;276(2):133–148. doi: 10.1007/BF00501186. [DOI] [PubMed] [Google Scholar]
  20. Smith R. W., Walsh A. Effect of lactation on lipolysis in rat adipose tissue. Lipids. 1976 May;11(5):418–420. doi: 10.1007/BF02532850. [DOI] [PubMed] [Google Scholar]
  21. Sollevi A., Fredholm B. B. The antilipolytic effect of endogenous and exogenous adenosine in canine adipose tissue in situ. Acta Physiol Scand. 1981 Sep;113(1):53–60. doi: 10.1111/j.1748-1716.1981.tb06861.x. [DOI] [PubMed] [Google Scholar]
  22. Steingrimsdottir L., Greenwood M. R., Brasel J. A. Effect of pregnancy, lactation and a high-fat diet on adipose tissue in Osborne-Mendel rats. J Nutr. 1980 Apr;110(4):600–609. doi: 10.1093/jn/110.4.600. [DOI] [PubMed] [Google Scholar]
  23. Vernon R. G. Development of perirenal adipose tissue in the neonatal lamb: effects of dietary safflower oil. Biol Neonate. 1977;32(1-2):15–23. doi: 10.1159/000240991. [DOI] [PubMed] [Google Scholar]
  24. Wang C., Smith R. L. Lowry determination of protein in the presence of Triton X-100. Anal Biochem. 1975 Feb;63(2):414–417. doi: 10.1016/0003-2697(75)90363-2. [DOI] [PubMed] [Google Scholar]
  25. Zinder O., Shapiro B. Effect of cell size on epinephrine- and ACTH-induced fatty acid release from isolated fat cells. J Lipid Res. 1971 Jan;12(1):91–95. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES