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. 1983 Dec 15;216(3):627–631. doi: 10.1042/bj2160627

Hormonal regulation of phosphatidylcholine synthesis in plants. The inhibition of cytidylyltransferase activity by indol-3-ylacetic acid.

M J Price-Jones, J L Harwood
PMCID: PMC1152555  PMID: 6320794

Abstract

Indol-3-ylacetic acid stimulated stem elongation within 1 h of treatment of Pisum sativum L. cv. Feltham First stem sections. This elongation was accompanied by an increase in the endogenous level of phosphocholine and a decrease in that of CDP-choline. Measurements in vitro of the CDP-base pathway enzymes showed an increase in choline phosphotransferase and a decrease in cytidylyltransferase activity on hormone treatment. These results indicate that the decrease in phosphatidylcholine labelling from [14C]choline that is observed on indol-3-ylacetic acid treatment of pea stem sections is caused by the decrease in cytidylyltransferase activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEISS U. ZUR PAPIERCHROMATOGRAPHISCHEN AUFTRENNUNG VON PFLANZENLIPIDEN. J Chromatogr. 1964 Jan;13:104–110. doi: 10.1016/s0021-9673(01)95079-4. [DOI] [PubMed] [Google Scholar]
  2. Barak A. J., Tuma D. J. Determination of choline, phosphorylcholine, and betaine. Methods Enzymol. 1981;72:287–292. doi: 10.1016/s0076-6879(81)72016-0. [DOI] [PubMed] [Google Scholar]
  3. Bolton P., Harwood J. L. Fatty acid biosynthesis by a particulate preparation from germinating pea. Biochem J. 1977 Nov 15;168(2):261–269. doi: 10.1042/bj1680261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Choy P. C., Lim P. H., Vance D. E. Purification and characterization of CTP: cholinephosphate cytidylytransferase from rat liver cytosol. J Biol Chem. 1977 Nov 10;252(21):7673–7677. [PubMed] [Google Scholar]
  5. Evans M. L., Ray P. M. Inactivity of 3-methyleneoxindole as mediator of auxin action on cell elongation. Plant Physiol. 1973 Aug;52(2):186–189. doi: 10.1104/pp.52.2.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Evins W. H., Varner J. E. Hormone-controlled synthesis of endoplasmic reticulum in barley aleurone cells. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1631–1633. doi: 10.1073/pnas.68.7.1631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feldman D. A., Kovac C. R., Dranginis P. L., Weinhold P. A. The role of phosphatidylglycerol in the activation of CTP:phosphocholine cytidylyltransferase from rat lung. J Biol Chem. 1978 Jul 25;253(14):4980–4986. [PubMed] [Google Scholar]
  8. Firn R. D., Kende H. Some effects of applied gibberellic Acid on the synthesis and degradation of lipids in isolated barley aleurone layers. Plant Physiol. 1974 Dec;54(6):911–915. doi: 10.1104/pp.54.6.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GARBUS J., DELUCA H. F., LOOMANS M. E., STRONG F. M. The rapid incorporation of phosphate into mitochondrial lipids. J Biol Chem. 1963 Jan;238:59–63. [PubMed] [Google Scholar]
  10. Harwood J. L. The synthesis of acyl lipids in plant tissues. Prog Lipid Res. 1979;18(2):55–86. doi: 10.1016/0163-7827(79)90006-7. [DOI] [PubMed] [Google Scholar]
  11. Infante J. P., Kinsella J. E. A novel method for determining equilibrium constants. CTP:phosphorylcholine cytidyltransferase. Biochim Biophys Acta. 1978 Oct 12;526(2):440–449. doi: 10.1016/0005-2744(78)90135-3. [DOI] [PubMed] [Google Scholar]
  12. Johnson K. D., Kende H. Hormonal Control of Lecithin Synthesis in Barley Aleurone Cells: Regulation of the CDP-Choline Pathway by Gibberellin. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2674–2677. doi: 10.1073/pnas.68.11.2674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Khosla S. S., Gobran L. I., Rooney S. A. Stimulation of phosphatidylcholine synthesis by 17 beta-estradiol in fetal rabbit lung. Biochim Biophys Acta. 1980 Feb 22;617(2):282–290. doi: 10.1016/0005-2760(80)90171-x. [DOI] [PubMed] [Google Scholar]
  14. Koehler D. E., Varner J. E. Hormonal control of orthophosphate incorporation into phospholipids of barley aleurone layers. Plant Physiol. 1973 Sep;52(3):208–214. doi: 10.1104/pp.52.3.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lord J. M. Evidence that phosphatidylcholine and phosphatidylethanolamine are synthesized by a single enzyme present in the endoplasmic reticulum of castor-bean endosperm. Biochem J. 1975 Nov;151(2):451–453. doi: 10.1042/bj1510451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miller J. C., Weinhold P. A. Cholinephosphotransferase in rat lung. The in vitro synthesis of dipalmitoylphosphatidylcholine from dipalmitoylglycerol. J Biol Chem. 1981 Dec 25;256(24):12662–12665. [PubMed] [Google Scholar]
  17. Mirbahar R. B., Laidman D. L. Gibberellic acid-stimulated alpha-amylase secretion and phospholipid metabolism in wheat aleurone tissue. Biochem J. 1982 Oct 15;208(1):93–100. doi: 10.1042/bj2080093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Montague M. J., Ray P. M. Phospholipid-synthesizing Enzymes Associated with Golgi Dictyosomes from Pea Tissue. Plant Physiol. 1977 Feb;59(2):225–230. doi: 10.1104/pp.59.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Morré D. J., Nyquist S., Rivera E. Lecithin Biosynthetic Enzymes of Onion Stem and the Distribution of Phosphorylcholine-Cytidyl Transferase among Cell Fractions. Plant Physiol. 1970 Jun;45(6):800–804. doi: 10.1104/pp.45.6.800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Oldenborg V., Van Golde L. M. The enzymes of phosphatidylcholine biosynthesis in the fetal mouse lung. Effects of dexamethasone. Biochim Biophys Acta. 1977 Dec 21;489(3):454–465. doi: 10.1016/0005-2760(77)90166-7. [DOI] [PubMed] [Google Scholar]
  21. TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]
  22. Tanaka K., Tolbert N. E. Effect of Cycocel derivatives and gibberellin on choline kinase and choline metabolism. Plant Physiol. 1966 Feb;41(2):313–318. doi: 10.1104/pp.41.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ulane R. E., Stephenson L. L., Farrell P. M. A rapid accurate assay for choline kinase. Anal Biochem. 1977 May 1;79(1-2):526–534. doi: 10.1016/0003-2697(77)90426-2. [DOI] [PubMed] [Google Scholar]

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