Abstract
The capacity of E. coli cells to regulate intracellular pH (pHi) during net potassium uptake has been investigated. The data show: (a) that cells sense their intracellular pH; (b) that the pH gradient (delta pH) exerts a feedback regulation on pHi; (c) that a mechanism of regulation of pHi exists which may be independent of Na+ [Zilberstein, Agmon, Schuldiner & Padan (1982) J. Biol. Chem. 257, 3687-3691]; and (d) that cells have a limited capacity to raise their intracellular pH in the absence of net K+ transport.
Full text
PDF![709](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84c6/1152566/a9b0cf6d7d82/biochemj00337-0183.png)
![710](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84c6/1152566/9c11acf36901/biochemj00337-0184.png)
![711](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84c6/1152566/7be5b6014998/biochemj00337-0185.png)
![712](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84c6/1152566/f09df2393b15/biochemj00337-0186.png)
![713](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84c6/1152566/5ee09e750694/biochemj00337-0187.png)
![714](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84c6/1152566/202c9661b124/biochemj00337-0188.png)
![715](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84c6/1152566/b4929e5aad1f/biochemj00337-0189.png)
![716](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84c6/1152566/04e9796b8597/biochemj00337-0190.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bakker E. P., Mangerich W. E. Interconversion of components of the bacterial proton motive force by electrogenic potassium transport. J Bacteriol. 1981 Sep;147(3):820–826. doi: 10.1128/jb.147.3.820-826.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Booth I. R., Kroll R. G. Regulation of cytoplasmic pH (pH1) in bacteria and its relationship to metabolism. Biochem Soc Trans. 1983 Jan;11(1):70–72. doi: 10.1042/bst0110070. [DOI] [PubMed] [Google Scholar]
- Booth I. R., Mitchell W. J., Hamilton W. A. Quantitative analysis of proton-linked transport systems. The lactose permease of Escherichia coli. Biochem J. 1979 Sep 15;182(3):687–696. doi: 10.1042/bj1820687. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brey R. N., Rosen B. P., Sorensen E. N. Cation/proton antiport systems in Escherichia coli. Properties of the potassium/proton antiporter. J Biol Chem. 1980 Jan 10;255(1):39–44. [PubMed] [Google Scholar]
- Collins S. H., Hamilton W. A. Magnitude of the protonmotive force in respiring Staphylococcus aureus and Escherichia coli. J Bacteriol. 1976 Jun;126(3):1224–1231. doi: 10.1128/jb.126.3.1224-1231.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Epstein W., Kim B. S. Potassium transport loci in Escherichia coli K-12. J Bacteriol. 1971 Nov;108(2):639–644. doi: 10.1128/jb.108.2.639-644.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Erecińska M., Deutsch C. J., Davis J. S. Energy coupling to K+ transport in Paracoccus denitrificans. J Biol Chem. 1981 Jan 10;256(1):278–284. [PubMed] [Google Scholar]
- Harold F. M., Papineau D. Cation transport and electrogenesis by Streptococcus faecalis. I. The membrane potential. J Membr Biol. 1972;8(1):27–44. doi: 10.1007/BF01868093. [DOI] [PubMed] [Google Scholar]
- Kitada M., Guffanti A. A., Krulwich T. A. Bioenergetic properties and viability of alkalophilic Bacillus firmus RAB as a function of pH and Na+ contents of the incubation medium. J Bacteriol. 1982 Dec;152(3):1096–1104. doi: 10.1128/jb.152.3.1096-1104.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kobayashi H., Murakami N., Unemoto T. Regulation of the cytoplasmic pH in Streptococcus faecalis. J Biol Chem. 1982 Nov 25;257(22):13246–13252. [PubMed] [Google Scholar]
- Kroll R. G., Booth I. R. The role of potassium transport in the generation of a pH gradient in Escherichia coli. Biochem J. 1981 Sep 15;198(3):691–698. doi: 10.1042/bj1980691. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krulwich T. A., Guffanti A. A., Bornstein R. F., Hoffstein J. A sodium requirement for growth, solute transport, and pH homeostasis in Bacillus firmus RAB. J Biol Chem. 1982 Feb 25;257(4):1885–1889. [PubMed] [Google Scholar]
- Krulwich T. A., Mandel K. G., Bornstein R. F., Guffanti A. A. A non-alkalophilic mutant of Bacillus alcalophilus lacks the Na+/H+ antiporter. Biochem Biophys Res Commun. 1979 Nov 14;91(1):58–62. doi: 10.1016/0006-291x(79)90582-5. [DOI] [PubMed] [Google Scholar]
- Padan E., Zilberstein D., Rottenberg H. The proton electrochemical gradient in Escherichia coli cells. Eur J Biochem. 1976 Apr 1;63(2):533–541. doi: 10.1111/j.1432-1033.1976.tb10257.x. [DOI] [PubMed] [Google Scholar]
- Padan E., Zilberstein D., Schuldiner S. pH homeostasis in bacteria. Biochim Biophys Acta. 1981 Dec;650(2-3):151–166. doi: 10.1016/0304-4157(81)90004-6. [DOI] [PubMed] [Google Scholar]
- Plack R. H., Jr, Rosen B. P. Cation/proton antiport systems in Escherichia coli. Absence of potassium/proton antiporter activity in a pH-sensitive mutant. J Biol Chem. 1980 May 10;255(9):3824–3825. [PubMed] [Google Scholar]
- Raven J. A., Smith F. A. The evolution of chemiosmotic energy coupling. J Theor Biol. 1976 Apr;57(2):301–312. doi: 10.1016/0022-5193(76)90003-5. [DOI] [PubMed] [Google Scholar]
- Rhoads D. B., Waters F. B., Epstein W. Cation transport in Escherichia coli. VIII. Potassium transport mutants. J Gen Physiol. 1976 Mar;67(3):325–341. doi: 10.1085/jgp.67.3.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanders D., Slayman C. L. Control of intracellular pH. Predominant role of oxidative metabolism, not proton transport, in the eukaryotic microorganism Neurospora. J Gen Physiol. 1982 Sep;80(3):377–402. doi: 10.1085/jgp.80.3.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schuldiner S., Fishkes H. Sodium-proton antiport in isolated membrane vesicles of Escherichia coli. Biochemistry. 1978 Feb 21;17(4):706–711. doi: 10.1021/bi00597a023. [DOI] [PubMed] [Google Scholar]
- Slonczewski J. L., Macnab R. M., Alger J. R., Castle A. M. Effects of pH and repellent tactic stimuli on protein methylation levels in Escherichia coli. J Bacteriol. 1982 Oct;152(1):384–399. doi: 10.1128/jb.152.1.384-399.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tokuda H., Nakamura T., Unemoto T. Potassium ion is required for the generation of pH-dependent membrane potential and delta pH by the marine bacterium Vibrio alginolyticus. Biochemistry. 1981 Jul 7;20(14):4198–4203. doi: 10.1021/bi00517a038. [DOI] [PubMed] [Google Scholar]
- Zilberstein D., Agmon V., Schuldiner S., Padan E. The sodium/proton antiporter is part of the pH homeostasis mechanism in Escherichia coli. J Biol Chem. 1982 Apr 10;257(7):3687–3691. [PubMed] [Google Scholar]