Fig. 4.

WA decreased LDs accumulation and inflammatory response induced by LPS. Microglia were pretreated with WA (10, 25, 50 µM) for 1 h. Subsequently, microglia were stimulated with LPS for 12 h to induce an inflammatory response. (A) Primary microglia were labeled with BODIPY fuorescence probe to visualize LDs molecules (scale bar = 5 μm). (B) Average fluorescence intensity of labeled LDs was quantified (Three independent replicate experiments were performed). (C) Primary microglia were subjected to Oil Red staining to assess LDs accumulation (scale bar = 5 μm). (D) Average area covered by labeled LDs on cells was determined (Four independent replicate experiments were performed). (E) Immunofluorescence staining of IL-1β was performed on primary microglia (scale bar = 10–5 μm), and the average fluorescence intensity of IL-1β is shown in (F) (Four independent replicate experiments were performed). *P < 0.05, **P < 0.01, ***P < 0.001 compared with the corresponding group, as determined by the one-way ANOVA .