Fig. 5.

WA promoted microglia autophagy and accelerated the degradation of LDs. Microglia were pre-treated with MG132 (10 µM) or 3-MA (5 mM) for one hour, followed by WA protection for one hour, and subsequently stimulated with LPS for 12 h. (A) BODIPY fluorescence probe was used to label LDs molecules in primary microglia (scale bar = 5 μm). (B) Average fluorescence intensity of labeled LDs was measured. (Four independent replicate experiments were conducted). (C) LDs accumulation was assessed in primary microglia through Oil Red staining (scale bar = 5 μm). (D Average area covered by labeled LDs on cells was determined (Four independent replicate experiments were conducted). (E) Primary microglia were labeled using a dual-fluorescence virus (GFP-RFP-MAP1LC3B) to visualize autophagosomes (scale bar = 5 μm). (F) Immunoblotting was performed to detect SQSTM1 and MAP1LC3B expression levels. Band intensities of SQSTM1 and MAP1LC3B in Western blots were quantified and presented in (G) and (H), respectively (Three independent replicate experiments were performed). *P < 0.05, **P < 0.01, ***P < 0.001 compared with the corresponding group, as determined by the one-way ANOVA .