Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 Aug 15;230(1):143–150. doi: 10.1042/bj2300143

The reaction of glutathione with the eye-lens protein gamma-crystallin.

C Slingsby, L Miller
PMCID: PMC1152597  PMID: 4052032

Abstract

Lens cells contain high concentrations of thiol-rich proteins, gamma-crystallins and reduced glutathione. Solutions of bovine gamma-crystallin react avidly with either reduced or oxidized glutathione to form protein-glutathione mixed disulphides. A method of purification of a gamma-II crystallin-glutathione adduct containing two mixed disulphide groups is described.

Full text

PDF
143

Images in this article

146Fig. 1.
on p.146
147Fig. 2.
on p.147
149Fig. 3.
on p.149
149Fig. 4.
on p.149

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akerboom T., Inoue M., Sies H., Kinne R., Arias I. M. Biliary transport of glutathione disulfide studied with isolated rat-liver canalicular-membrane vesicles. Eur J Biochem. 1984 May 15;141(1):211–215. doi: 10.1111/j.1432-1033.1984.tb08177.x. [DOI] [PubMed] [Google Scholar]
  2. Anderson E. I., Spector A. The state of sulfhydryl groups in normal and cataractous human lens proteins. I. Nuclear region. Exp Eye Res. 1978 Apr;26(4):407–417. doi: 10.1016/0014-4835(78)90128-8. [DOI] [PubMed] [Google Scholar]
  3. BJORK I. Studies on gamma-crystallin from calf lens. I. Isolation by gel filtration. Exp Eye Res. 1961 Dec;1:145–154. doi: 10.1016/s0014-4835(61)80020-1. [DOI] [PubMed] [Google Scholar]
  4. Bhat S. P., Spector A. Complete nucleotide sequence of a cDNA derived from calf lens gamma-crystallin mRNA: presence of Alu I-like DNA sequences. DNA. 1984 Aug;3(4):287–295. doi: 10.1089/dna.1.1984.3.287. [DOI] [PubMed] [Google Scholar]
  5. Blundell T., Lindley P., Miller L., Moss D., Slingsby C., Tickle I., Turnell B., Wistow G. The molecular structure and stability of the eye lens: x-ray analysis of gamma-crystallin II. Nature. 1981 Feb 26;289(5800):771–777. doi: 10.1038/289771a0. [DOI] [PubMed] [Google Scholar]
  6. Harding J. J., Dilley K. J. Structural proteins of the mammalian lens: a review with emphasis on changes in development, aging and cataract. Exp Eye Res. 1976 Jan;22(1):1–73. doi: 10.1016/0014-4835(76)90033-6. [DOI] [PubMed] [Google Scholar]
  7. Harding J. J. Free and protein-bound glutathione in normal and cataractous human lenses. Biochem J. 1970 May;117(5):957–960. doi: 10.1042/bj1170957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KINOSHITA J. H., MEROLA L. O. The reactivity of sulfhydryl groups in bovine lenses. Arch Biochem Biophys. 1959 Apr;81(2):395–403. doi: 10.1016/0003-9861(59)90218-8. [DOI] [PubMed] [Google Scholar]
  9. KINOSHITA J. H. SELECTED TOPICS IN OPHTHALMIC BIOCHEMISTRY. Arch Ophthalmol. 1964 Oct;72:554–572. doi: 10.1001/archopht.1964.00970020554022. [DOI] [PubMed] [Google Scholar]
  10. Kuck J. F., Yu N. T., Askren C. C. Total sulfhydryl by raman spectroscopy in the intact lens of several species: variations in the nucleus and along the optical axis during aging. Exp Eye Res. 1982 Jan;34(1):23–37. doi: 10.1016/0014-4835(82)90005-7. [DOI] [PubMed] [Google Scholar]
  11. Lok S., Tsui L. C., Shinohara T., Piatigorsky J., Gold R., Breitman M. Analysis of the mouse gamma-crystallin gene family: assignment of multiple cDNAs to discrete genomic sequences and characterization of a representative gene. Nucleic Acids Res. 1984 Jun 11;12(11):4517–4529. doi: 10.1093/nar/12.11.4517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meister A., Anderson M. E. Glutathione. Annu Rev Biochem. 1983;52:711–760. doi: 10.1146/annurev.bi.52.070183.003431. [DOI] [PubMed] [Google Scholar]
  13. Moormann R. J., den Dunnen J. T., Bloemendal H., Schoenmakers J. G. Extensive intragenic sequence homology in two distinct rat lens gamma-crystallin cDNAs suggests duplications of a primordial gene. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6876–6880. doi: 10.1073/pnas.79.22.6876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rathbun W. B., Sethna S. S., Skelnik D. L., Bistner S. I. Glutathione metabolism in lenses of dogs and rabbits: activities of five enzymes. Exp Eye Res. 1983 Jun;36(6):845–858. doi: 10.1016/0014-4835(83)90038-6. [DOI] [PubMed] [Google Scholar]
  15. Reddy D. V., Klethi J., Kinsey V. E. Studies on the crystalline lens. XII. Turnover of glycine and glutamic acid in glutathione and ophthalmic acid in the rabbit. Invest Ophthalmol. 1966 Dec;5(6):594–600. [PubMed] [Google Scholar]
  16. Schoenmakers J. G., den Dunnen J. T., Moormann R. J., Jongbloed R., van Leen R. W., Lubsen N. H. The crystallin gene families. Ciba Found Symp. 1984;106:208–218. doi: 10.1002/9780470720875.ch12. [DOI] [PubMed] [Google Scholar]
  17. Slingsby C., Croft L. R. Structural studies on calf lens gamma-crystallin fraction IV: a comparison of the cysteine-containing tryptic peptides with the corresponding amino acid sequence of gamma-crystallin fraction II. Exp Eye Res. 1978 Mar;26(3):291–304. doi: 10.1016/0014-4835(78)90076-3. [DOI] [PubMed] [Google Scholar]
  18. Slingsby C., Miller L. R. Purification and crystallization of mammalian lens gamma-crystallins. Exp Eye Res. 1983 Nov;37(5):517–530. doi: 10.1016/0014-4835(83)90028-3. [DOI] [PubMed] [Google Scholar]
  19. Spector A. Oxidation and cataract. Ciba Found Symp. 1984;106:48–64. doi: 10.1002/9780470720875.ch4. [DOI] [PubMed] [Google Scholar]
  20. Srivastava S. K., Ansari N. H., Awasthi Y. C. Lens glutathione depletion of 1-chloro-2,4-dinitrobenzene and oxidative stress. Curr Eye Res. 1984 Jan;3(1):117–119. doi: 10.3109/02713688408997192. [DOI] [PubMed] [Google Scholar]
  21. Srivastava S. K., Beutler E. Cleavage of lens protein-GSH mixed disulfide by glutathione reductase. Exp Eye Res. 1973 Oct 10;17(1):33–42. doi: 10.1016/0014-4835(73)90165-6. [DOI] [PubMed] [Google Scholar]
  22. Summers L., Slingsby C., White H., Narebor M., Moss D., Miller L., Mahadevan D., Lindley P., Driessen H., Blundell T. The molecular structures and interactions of bovine and human gamma-crystallins. Ciba Found Symp. 1984;106:219–236. doi: 10.1002/9780470720875.ch13. [DOI] [PubMed] [Google Scholar]
  23. Tomarev S. I., Zinovieva R. D., Chalovka P., Krayev A. S., Skryabin K. G., Gause G. G., Jr Multiple genes coding for the frog eye lens gamma-crystallins. Gene. 1984 Mar;27(3):301–308. doi: 10.1016/0378-1119(84)90074-x. [DOI] [PubMed] [Google Scholar]
  24. Wistow G., Turnell B., Summers L., Slingsby C., Moss D., Miller L., Lindley P., Blundell T. X-ray analysis of the eye lens protein gamma-II crystallin at 1.9 A resolution. J Mol Biol. 1983 Oct 15;170(1):175–202. doi: 10.1016/s0022-2836(83)80232-0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES