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. 1985 Oct 1;231(1):105–113. doi: 10.1042/bj2310105

Affinity purification and refined structural characterization of terminal deoxynucleotidyltransferase.

S A Fuller, A Philips, M S Coleman
PMCID: PMC1152709  PMID: 3904729

Abstract

A total of 56 stable murine hybridoma monoclones that produce homogeneous antibodies against human or calf terminal deoxynucleotidyltransferase have been established. All of the antibodies exhibited specific binding to various Mr forms of terminal transferase and eight possessed neutralizing activity. Results are presented that permitted characterization of ten of these antibodies with respect to their immunoglobulin class, their recognition of calf or human terminal-transferase Mr species by immunoblotting techniques and their recognition of distinct antigenic sites. Terminal transferase was purified in a single step by using an immunoaffinity column constructed with a monoclonal antibody exhibiting a high binding affinity for the enzyme. Single monoclonal antibodies were also used to bind selectively to terminal-transferase antigen in tissue slices and individual cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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