Table 2.

Calculated changes in free energy and binding affinities for the original target DNA and the mismatches.

DNA	$\text{Δ}G$ (Kcal/mol)	$\text{Δ}G/RT$	$K_d(\exp (-\text{Δ}G/RT))$[M]
Target	56.8	95.4	3.7 × 10−42
mmP1	41.5	69.7	5.4 × 10−31
mmP2	36.8	61.8	1.4 × 10−27
mmP1P2	21.5	36.1	2.1 × 10−16