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. 1985 Oct 1;231(1):229–232. doi: 10.1042/bj2310229

A new site-specific endonuclease, ScaI, from Streptomyces caespitosus.

H Takahashi, H Kojima, H Saito
PMCID: PMC1152731  PMID: 2998344

Abstract

A new site-specific endonuclease has been isolated from Streptomyces caespitosus and named ScaI. Based on analysis of sequences around the restriction sites in pBR322 and pBR325, the recognition sequence of ScaI endonuclease was deduced to be a new hexanucleotide 5'-AGTACT-3'. The cleavage site was determined by comparing the ScaI-cleaved product of a primer-extended M13mp18-SCA DNA, which contains an AGTACT sequence, with dideoxy chain terminator ladders of the same DNA. ScaI was found to cleave the recognition sequence between the internal T and A, leaving flush ends to the cleaved fragments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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