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. 1985 Oct 15;231(2):375–382. doi: 10.1042/bj2310375

Isolation and characterization of six human hepatic isometallothioneins.

P E Hunziker, J H Kägi
PMCID: PMC1152756  PMID: 4062903

Abstract

Human hepatic metallothionein (MT) was separated into six isoforms by using reversed-phase h.p.l.c. at the analytical and preparative levels. By comparison with the h.p.l.c. elution profiles of the charge-separable species MT-1 and MT-2 isolated by the procedure of Bühler & Kägi [(1974) FEBS Lett. 39, 229-234], five of these isoproteins are identified as hitherto unresolved subforms of MT-1, and one is identical with MT-2. The six isoforms have distinct and reproducible retention times at neutral pH, where the metal remains bound to the protein, and at low pH, where the metal is removed. Their amino acid compositions display the high cysteine content and the lack of aromatic amino acids and of histidine typical of mammalian metallothioneins, but they differ significantly with respect to all other amino acids. A survey of autopsy material indicates that in adult human liver all six isoforms are usually expressed, albeit in somewhat variable relative proportions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bühler R. H., Kägi J. H. Human hepatic metallothioneins. FEBS Lett. 1974 Feb 15;39(2):229–234. doi: 10.1016/0014-5793(74)80057-8. [DOI] [PubMed] [Google Scholar]
  2. Etzel K. R., Cousins R. J. Hormonal regulation of liver metallothionein zinc: independent and synergistic action of glucagon and glucocorticoids. Proc Soc Exp Biol Med. 1981 Jun;167(2):233–236. doi: 10.3181/00379727-167-41155. [DOI] [PubMed] [Google Scholar]
  3. Huang I. Y., Kimura M., Hata A., Tsunoo H., Yoshida A. Complete amino acid sequence of mouse liver metallothionein-II. J Biochem. 1981 Jun;89(6):1839–1845. doi: 10.1093/oxfordjournals.jbchem.a133385. [DOI] [PubMed] [Google Scholar]
  4. Huang I. Y., Yoshida A. Mouse liver metallothioneins. Complete amino acid sequence of metallothionein-I. J Biol Chem. 1977 Nov 25;252(22):8217–8221. [PubMed] [Google Scholar]
  5. Karin M., Richards R. I. Human metallothionein genes--primary structure of the metallothionein-II gene and a related processed gene. Nature. 1982 Oct 28;299(5886):797–802. doi: 10.1038/299797a0. [DOI] [PubMed] [Google Scholar]
  6. Karin M., Richards R. I. Human metallothionein genes: molecular cloning and sequence analysis of the mRNA. Nucleic Acids Res. 1982 May 25;10(10):3165–3173. doi: 10.1093/nar/10.10.3165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kissling M. M., Kägi H. R. Primary structure of human hepatic metallothionein. FEBS Lett. 1977 Oct 15;82(2):247–250. doi: 10.1016/0014-5793(77)80594-2. [DOI] [PubMed] [Google Scholar]
  8. Kissling M. M., Kägi J. H. Amino acid sequence of human hepatic metallothioneins. Experientia Suppl. 1979;34:145–151. doi: 10.1007/978-3-0348-6493-0_5. [DOI] [PubMed] [Google Scholar]
  9. Klauser S., Kägi J. H., Wilson K. J. Characterization of isoprotein patterns in tissue extracts and isolated samples of metallothioneins by reverse-phase high-pressure liquid chromatography. Biochem J. 1983 Jan 1;209(1):71–80. doi: 10.1042/bj2090071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oh S. H., Deagen J. T., Whanger P. D., Weswig P. H. Biological function of metallothionein. V. Its induction in rats by various stresses. Am J Physiol. 1978 Mar;234(3):E282–E285. doi: 10.1152/ajpendo.1978.234.3.E282. [DOI] [PubMed] [Google Scholar]
  11. Otvos J. D., Armitage I. M. Structure of the metal clusters in rabbit liver metallothionein. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7094–7098. doi: 10.1073/pnas.77.12.7094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Richards R. I., Heguy A., Karin M. Structural and functional analysis of the human metallothionein-IA gene: differential induction by metal ions and glucocorticoids. Cell. 1984 May;37(1):263–272. doi: 10.1016/0092-8674(84)90322-2. [DOI] [PubMed] [Google Scholar]
  13. Sobocinski P. Z., Canterbury W. J., Jr, Mapes C. A., Dinterman R. E. Involvement of hepatic metallothioneins in hypozincemia associated with bacterial infection. Am J Physiol. 1978 Apr;234(4):E399–E406. doi: 10.1152/ajpendo.1978.234.4.E399. [DOI] [PubMed] [Google Scholar]

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