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. 1985 Nov 1;231(3):743–753. doi: 10.1042/bj2310743

Electron transfer to nitrogenase in Klebsiella pneumoniae. nifF gene cloned and the gene product, a flavodoxin, purified.

J Deistung, F C Cannon, M C Cannon, S Hill, R N Thorneley
PMCID: PMC1152811  PMID: 3907625

Abstract

The nifF gene of Klebsiella pneumoniae was cloned into a multicopy plasmid in order to construct a strain that synthesizes and retains an elevated concentration of the gene product relative to the wild-type strain. Characterization of the isolated flavodoxin, which serves as an electron donor to nitrogenase, shows unambiguously that it is the product of the nifF gene.

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Selected References

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