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. 1985 Nov 15;232(1):93–98. doi: 10.1042/bj2320093

Biosynthesis of complement components by cultured rat hepatocytes.

R Anthony, L Morrison, R N MacSween, K Whaley
PMCID: PMC1152844  PMID: 3853462

Abstract

Hepatocytes which had been isolated from the livers of Charles River rats were cultured in vitro. The cells were shown to synthesize albumin and the complement components C4, C2, C3 and B. Pulse-label studies with [35S]methionine showed that C4 and C3 were synthesized as single polypeptide chains. Pro-C4 did not appear to be converted into the plasma form of C4 intracellularly, whereas cell lysates contained the alpha- and beta-chains of plasma C3 as well as pro-C3. It is concluded that culture of rat hepatocytes in vitro provides a useful technique for studies of the synthesis of complement components.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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