Abstract
Individuals whose erythrocytes are positive for the rare blood-group antigen Webb (Wb) have an altered form of the minor sialoglycoprotein beta (synonyms glycophorin C and glycoconnectin). This altered sialoglycoprotein beta (beta Wb) has an Mr about 2700 lower than that of normal sialoglycoprotein beta. Treatment of normal sialoglycoprotein beta with endo-beta-N-acetylglucosaminidase F decreased its Mr by about 3600, but similar treatment of sialoglycoprotein beta Wb had no effect. These results suggest the possibility that sialoglycoprotein beta Wb lacks the N-glycosidically linked oligosaccharide found on normal sialoglycoprotein beta.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anstee D. J., Mawby W. J., Tanner M. J. Abnormal blood-group-Ss-active sialoglycoproteins in the membrane of Miltenberger class III, IV and V human erythrocytes. Biochem J. 1979 Nov 1;183(2):193–203. doi: 10.1042/bj1830193. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anstee D. J., Parsons S. F., Ridgwell K., Tanner M. J., Merry A. H., Thomson E. E., Judson P. A., Johnson P., Bates S., Fraser I. D. Two individuals with elliptocytic red cells apparently lack three minor erythrocyte membrane sialoglycoproteins. Biochem J. 1984 Mar 1;218(2):615–619. doi: 10.1042/bj2180615. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anstee D. J., Ridgwell K., Tanner M. J., Daniels G. L., Parsons S. F. Individuals lacking the Gerbich blood-group antigen have alterations in the human erythrocyte membrane sialoglycoproteins beta and gamma. Biochem J. 1984 Jul 1;221(1):97–104. doi: 10.1042/bj2210097. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dahr W., Beyreuther K., Kordowicz M., Krüger J. N-terminal amino acid sequence of sialoglycoprotein D (glycophorin C) from human erythrocyte membranes. Eur J Biochem. 1982 Jun 15;125(1):57–62. doi: 10.1111/j.1432-1033.1982.tb06650.x. [DOI] [PubMed] [Google Scholar]
- Dahr W., Newman R. A., Contreras M., Kordowicz M., Teesdale P., Beyreuther K., Krüger J. Structures of Miltenberger class I and II specific major human erythrocyte membrane sialoglycoproteins. Eur J Biochem. 1984 Jan 16;138(2):259–265. doi: 10.1111/j.1432-1033.1984.tb07910.x. [DOI] [PubMed] [Google Scholar]
- Elder J. H., Alexander S. endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4540–4544. doi: 10.1073/pnas.79.15.4540. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lewis M., Allen F. H., Jr, Anstee D. J., Bird G. W., Brodheim E., Contreras M., Crookston M., Dahr W., Engelfriet C. P., Giles C. M. ISBT Working Party on Terminology for Red Cell Surface Antigens: Munich report. Vox Sang. 1985;49(2):171–175. doi: 10.1111/j.1423-0410.1985.tb00788.x. [DOI] [PubMed] [Google Scholar]
- Plummer T. H., Jr, Elder J. H., Alexander S., Phelan A. W., Tarentino A. L. Demonstration of peptide:N-glycosidase F activity in endo-beta-N-acetylglucosaminidase F preparations. J Biol Chem. 1984 Sep 10;259(17):10700–10704. [PubMed] [Google Scholar]
- SIMMONS R. T., ALBREY J. A. A "new" blood group antigen Webb (Wb) of low frequency found in two Australian families. Med J Aust. 1963 Jan 5;1(1):8–10. doi: 10.5694/j.1326-5377.1963.tb26536.x. [DOI] [PubMed] [Google Scholar]
- Tanner M. J., Anstee D. J., Mawby W. J. A new human erythrocyte variant (Ph) containing an abnormal membrane sialoglycoprotein. Biochem J. 1980 May 1;187(2):493–500. doi: 10.1042/bj1870493. [DOI] [PMC free article] [PubMed] [Google Scholar]