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. 1985 Dec 1;232(2):379–383. doi: 10.1042/bj2320379

Characteristics of lipopolysaccharide interaction with human peripheral-blood monocytes.

S J Warner, N Savage, D Mitchell
PMCID: PMC1152890  PMID: 3911946

Abstract

The interaction between radioiodinated lipopolysaccharide from Escherichia coli 0111:B4 (125I-LPS) and human peripheral-blood monocytes was studied. The association of 125I-LPS with monocytes at 37 degrees C appeared to depend on binding to the cell membrane with subsequent internalization of the molecule, and was not saturable with time (up to 2 h) or 125I-LPS concentration (up to 10 micrograms/ml). There was no apparent difference in the behaviour of unlabelled LPS and 125I-LPS with respect to monocyte association. 125I-LPS association with monocytes was inhibited by LPS and O-polysaccharide from E. coli 0111:B4 and Salmonella typhi 0901, but not by lipid A or polymyxin B. We propose that the mechanism of human monocyte stimulation by LPS involves polysaccharide-dependent binding to the cell membrane followed by internalization of the LPS molecule. We were unable to demonstrate a specific LPS receptor such as that found on murine B-lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ciznár I., Shands J. W., Jr Effect of alkali-treated lipopolysaccharide on erythrocyte membrane stability. Infect Immun. 1971 Oct;4(4):362–367. doi: 10.1128/iai.4.4.362-367.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coutinho A., Forni L., Watanabe T. Genetic and functional characterization of an antiserum to the lipid A-specific triggering receptor on murine B lymphocytes. Eur J Immunol. 1978 Jan;8(1):63–67. doi: 10.1002/eji.1830080113. [DOI] [PubMed] [Google Scholar]
  3. Dancey G. F., Yasuda T., Kinsky S. C. Enhancement of liposomal model membrane immunogenicity by incorporation of lipid A. J Immunol. 1977 Dec;119(6):1868–1873. [PubMed] [Google Scholar]
  4. Davies M., Stewart-Tull D. E., Jackson D. M. The binding of lipopolysaccharide from Escherichia coli to mammalian cell membranes and its effect on liposomes. Biochim Biophys Acta. 1978 Apr 4;508(2):260–276. doi: 10.1016/0005-2736(78)90329-2. [DOI] [PubMed] [Google Scholar]
  5. Davies M., Stewart-Tull D. E. The affinity of bacterial polysaccharide-containing fractions for mammalian cell membranes and its relationship to immunopotentiating activity. Biochim Biophys Acta. 1981 Apr 22;643(1):17–29. doi: 10.1016/0005-2736(81)90215-7. [DOI] [PubMed] [Google Scholar]
  6. De Mulder P. H., Wessels J. M., Rosenbrand D. A., Smeulders J. B., Wagener D. J., Haanen C. Monocyte purification with counterflow centrifugation monitored by continuous flow cytometry. J Immunol Methods. 1981;47(1):31–38. doi: 10.1016/0022-1759(81)90254-4. [DOI] [PubMed] [Google Scholar]
  7. Dinarello C. A. Interleukin-1. Rev Infect Dis. 1984 Jan-Feb;6(1):51–95. doi: 10.1093/clinids/6.1.51. [DOI] [PubMed] [Google Scholar]
  8. Galanos C., Lüderitz O. Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms. Eur J Biochem. 1975 Jun;54(2):603–610. doi: 10.1111/j.1432-1033.1975.tb04172.x. [DOI] [PubMed] [Google Scholar]
  9. Gimber P. E., Rafter G. W. The interaction of Escherichia coli endotoxin with leukocytes. Arch Biochem Biophys. 1969 Dec;135(1):14–20. doi: 10.1016/0003-9861(69)90510-4. [DOI] [PubMed] [Google Scholar]
  10. Goldman R. C., Leive L. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur J Biochem. 1980;107(1):145–153. doi: 10.1111/j.1432-1033.1980.tb04635.x. [DOI] [PubMed] [Google Scholar]
  11. Goodman S. A., Vukajlovich S. W., Munkenbeck P., Morrison D. C. Selective interaction between lymphocytes and lipid A subunits in lipopolysaccharide macromolecular aggregates. Rev Infect Dis. 1984 Jul-Aug;6(4):511–518. doi: 10.1093/clinids/6.4.511. [DOI] [PubMed] [Google Scholar]
  12. Gregory S. H., Zimmerman D. H., Kern M. 125I-lipid A binding by individual macrophage cells: cell purification, culture, and autoradiographic analysis on a single surface. Anal Biochem. 1980 Sep 1;107(1):246–250. doi: 10.1016/0003-2697(80)90517-5. [DOI] [PubMed] [Google Scholar]
  13. Haeffner-Cavaillon N., Chaby R., Cavaillon J. M., Szabó L. Lipopolysaccharide receptor on rabbit peritoneal macrophages. I. Binding characteristics. J Immunol. 1982 May;128(5):1950–1954. [PubMed] [Google Scholar]
  14. Hämmerling U., Westphal O. Synthesis and use of O-stearoyl polysaccharides in passive hemagglutination and hemolysis. Eur J Biochem. 1967 Mar;1(1):46–50. doi: 10.1007/978-3-662-25813-2_9. [DOI] [PubMed] [Google Scholar]
  15. Jacobs D. M., Roberts D. B., Eldridge J. H., Rosenspire A. J. Binding of bacterial lipopolysaccharide to murine lymphocytes. Ann N Y Acad Sci. 1983 Jun 30;409:72–81. doi: 10.1111/j.1749-6632.1983.tb26860.x. [DOI] [PubMed] [Google Scholar]
  16. Jann B., Reske K., Jann K. Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Eur J Biochem. 1975 Dec 1;60(1):239–246. doi: 10.1111/j.1432-1033.1975.tb20996.x. [DOI] [PubMed] [Google Scholar]
  17. Kabir S., Rosenstreich D. L. Binding of bacterial endotoxin to murine spleen lymphocytes. Infect Immun. 1977 Jan;15(1):156–164. doi: 10.1128/iai.15.1.156-164.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Larsen N. E., Sullivan R. Interaction between endotoxin and human monocytes: characteristics of the binding of 3H-labeled lipopolysaccharide and 51Cr-labeled lipid A before and after the induction of endotoxin tolerance. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3491–3495. doi: 10.1073/pnas.81.11.3491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Liang-Takasaki C. J., Saxén H., Mäkelä P. H., Leive L. Complement activation by polysaccharide of lipopolysaccharide: an important virulence determinant of salmonellae. Infect Immun. 1983 Aug;41(2):563–569. doi: 10.1128/iai.41.2.563-569.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lüderitz O., Tanamoto K., Galanos C., McKenzie G. R., Brade H., Zähringer U., Rietschel E. T., Kusumoto S., Shiba T. Lipopolysaccharides: structural principles and biologic activities. Rev Infect Dis. 1984 Jul-Aug;6(4):428–431. doi: 10.1093/clinids/6.4.428. [DOI] [PubMed] [Google Scholar]
  22. Mergenhagen S. E., Pluznik D. H. Defective responses to lipid A in C3H/HeJ mice: approaches to an understanding of lipid A-cell interaction. Rev Infect Dis. 1984 Jul-Aug;6(4):519–523. doi: 10.1093/clinids/6.4.519. [DOI] [PubMed] [Google Scholar]
  23. Morrison D. C., Jacobs D. M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry. 1976 Oct;13(10):813–818. doi: 10.1016/0019-2791(76)90181-6. [DOI] [PubMed] [Google Scholar]
  24. Morrison D. C., Leive L. Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures. J Biol Chem. 1975 Apr 25;250(8):2911–2919. [PubMed] [Google Scholar]
  25. Morrison D. C., Ulevitch R. J. The effects of bacterial endotoxins on host mediation systems. A review. Am J Pathol. 1978 Nov;93(2):526–618. [PMC free article] [PubMed] [Google Scholar]
  26. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  27. Raichvarg D., Hatat D., Sarfati G., Agneray J. Action of a polysaccharide fraction of Haemophilus influenzae lipopolysaccharide on macrophage: implication of receptor for mannosyl-polysaccharides. Med Microbiol Immunol. 1982;171(2):91–97. doi: 10.1007/BF02124916. [DOI] [PubMed] [Google Scholar]
  28. Symons D. B., Clarkson C. A. The binding of LPS to the lymphocyte surface. Immunology. 1979 Nov;38(3):503–508. [PMC free article] [PubMed] [Google Scholar]
  29. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  30. Ulevitch R. J. The preparation and characterization of a radioiodinated bacterial lipopolysaccharide. Immunochemistry. 1978 Mar;15(3):157–164. doi: 10.1016/0161-5890(78)90144-x. [DOI] [PubMed] [Google Scholar]
  31. Watson J., Riblet R. Genetic control of responses to bacterial lipopolysaccharides in mice. II. A gene that influences a membrane component involved in the activation of bone marrow-derived lymphocytes by lipipolysaccharides. J Immunol. 1975 May;114(5):1462–1468. [PubMed] [Google Scholar]
  32. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

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